elsa
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A 54 years old woman has arthritis,nephritis fever and a butterfly rash across her nose and cheeks.She complains of weight loss and fatique. Immunoflorescence studies show the presence of autoantibodies to U1 snRNP.WHich of the following processes is most likely to be impaired in this patient?
A. Assembly of th spliceosome
B. Binding to the branch site
C. Cytoplasmic transport of RNA
D. Recognition of the 3' splice site
E. Recognition of the 5' splice site
2.Investigators are studying the regulation of glycolytic pathway enzymes in a culture of hepatocytes. After citrate is added to the culture,a substance of which of the following enzyme catalyzed reactions will accumulate in the hepatocytes?
A. 1,3 bisphosphoglycerate------ 3 phosphoglycerate
B. Fructose 6 phosphate --------- fructose 1,6 biphosphate
C. Glucose-------------------------glucose 6 phosphate
D. glucose 6 phosphate---------fructose 6 phosphate
E. phosphoenolpyruvate--------pyruvate
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elsa
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| intern_doc
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1. A (snRNP found in nucleus of eukaryotes participates in splicing (removal of introns) mRNA
2. D (citrate is an inhibitor of PFK-1)
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| Idiopathic
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1) A
2) A
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| Malaysian
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my guess are
1)A
2)B
could be mistaken as my biochemistry is a bit rusty these days.but my point of argument is(if my facts are right)A is that snRNP is coding for the rRNA.....the ribosome which is used for assembly of protein
and B because PFK is a rate limiting enzyme which is inhibited by ATP and citrate.
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| Malaysian
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What is the full form of snRNP???I failed to find it in my biochemistry notes....the closest to it was RNAP...
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| sk
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1.A
2.B
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| mash
Forum Fanatic
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1 A
2 D
citrate inhibits PFK1
fructose 6 P will accumulate
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| elsa
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Guys I myself didnot encounter this on my readings I will give to you the answer and explanation as it is explained in Rapid Review by E. Goljan. If you have additional information to make it easy for me to understand it better please share it to me. And I will give you also the reasons why some of the choices are incorrect
1. The answer is E Recognition of the 5' splice site. The patient clinically and laboratory findings are consistent of SLE. SLE is a chronic immune disorder characterized by multisystem involvement and by clinical exacerbations and remission. Loss of tolerance to autoantibodies is the central pathogenesis of SLE. Autoantibodies can be formed to DNA-RNA hybrids,ribosomal subunits and the U1 small nuclear ribonucleoprotein particle ( U1snRNP) U1 snRNP takes part in splicing of primary transcripts and it is the snRNP that recognizes the 5' splice site.
A is incorrect because the spiceosome consist of U4-U6 snRNA and mRNA.
B is incorrect bevause U2 snRNP binds to the branch site and polypyrimidine tract.
C. is incorrect because U1 snRNP is found in the nucleus where the splicing takes place and has no influence on the RNA cytoplasmic transport.
D. is incorrect because U5 snRNP rogonizes the 3' splice site.
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elsa
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| elsa
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Malaysian and sk you are right the answer is B fructose 6 phosphate----fructose 1,6 biphosphate
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elsa
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