shawn333 Forum Senior
Topics: 25 Posts: 105
| | 05/09/04 - 02:52 AM  
 
   
 
|   #1 |
parietal cells secrete HCl then how come they are acidophilic? :?:
___________________ Time Wounds all Heals , Contain the damage .
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| peekay Forum Guru
Topics: 102 Posts: 588
| | 05/09/04 - 04:48 PM  
 
   
 
|   #2 |
i don't think what cell secrete ha something to do with how they stain. staining is due to cell wall properties
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| Sakaki- Forum Senior
Topics: 1 Posts: 238
| | 05/09/04 - 07:04 PM  
 
   
 
|   #3 |
Since the cytoplasm is eosinophilic, I thought that it was more likely the contents of the cytoplasm (whatever they may be) that determines the staining qualities. Anyways, I think that it is the organic material that stains, and perhaps not the HCl.
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| shawn333 Forum Senior
Topics: 25 Posts: 105
| | 05/10/04 - 01:30 PM  
 
   
 
|   #4 |
staining is due to the cytoplasm properties , not the cell wall . the cells making more proteins are acidophilic . anyway , thanks all for effort . really appreciate it .
___________________ Time Wounds all Heals , Contain the damage .
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| peekay Forum Guru
Topics: 102 Posts: 588
| | 05/10/04 - 03:56 PM  
 
   
 
|   #5 |
thanks for your effort every one
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| endrit Forum Newbie
Topics: 1 Posts: 11
| | 05/12/04 - 10:43 PM  
 
   
 
|   #6 |
the HCl is secreted outside the cell so there is no reason it should affect the stain.what is in the cell is probably more HCO3.
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| waqastariq
| | 06/17/06 - 11:11 PM  
 
   
 
|   #7 |
well the cells making a lot of protein should be basophilic...thats what my histo concepts tell me as I remmeber RNA is more and that is acid...so basophilic cytoplasm...
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| mildus Forum Guru
Topics: 19 Posts: 614
| | 06/20/06 - 01:28 AM  
 
   
 
|   #8 |
Parietal cells secrete H+ . This process requires energy (ATP) which is produced in mitochondria, that is why there are many mitochondria in parietal cells. Mitochondria contain much H+ (which is positively charged). So, mitochondria are acidophilic which means they stain with acidic stains that are negatively charged). This is the reason why parietal cells are acidophilic. On the other hand, chief cells are basophilic because their role is to produce enzyme so they have many ribosomes on the surface of endoplasmatic reticulum. Ribosomes, as well as the nucleus, stain with basic stains (basophilic) because they contain negatively charged nucleic acids which react with positively charged basic stains.
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