sujju
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what r all hormones secreted by acidophils of pitutary
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| rida
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ACTH, PRL, and GH
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| mjl1717
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This is kind of hi yield some times they ask about the the acidophils and basophils in the pit. what they stain with and what they do. Comments?
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| Sakaki-
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The acidophils consist of somatotrophs and lactotrophs, whereas the basophils consist of thyrotrophs, gonadotrophs, and corticotrophs.
As for what they stain with, I'm not quite sure. Acidophils stain with acidic dyes, and basophils stain with basic dyes. : I know that a PAS with an orange G counterstain will give quite good differentiation of the two cell types.
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| piter
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Eosin-acidic dye - is pinkish-orange - GH, prolactin
Hematoxylin - base dye - is purple-blue - ACTH, FSH, LH, TSH
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| rida
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piter isn't ACTH acidic???
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| piter
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100% base 
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| rida
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thanx!!
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| Sakaki-
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Heh--it's quite difficult to distinguish acidophils from basophils on a routine H&E stain.  But basically, any acidic dyes such as acid fuchsin will stain acidophils, and any basic dyes (ex. Schiff reagent) would stain basophils.
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