mjl1717
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Staph aureus produces NAD/ Haemophilus needs X (protoporphin) and V
(NAD) for growth[So the Haemophilus influenza grows around Staph aureus] --analagous to Denzel Washington in the middle and women of all sizes and shapes and colors conjugating around him
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Smell the coffee! "Is That an Osler move??"
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| mash
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gr8 analogy!
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I hear and I forget. I see and I remember. I do and I understand.
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| kalsam
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 good explanation..
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| bactitech
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The way to do this in the micro lab is to streak H. influenzae on a BAP (blood agar plate). Then, take Staph. aureus and inoculate across the HFLU in a straight line and incubate at 35 degrees C in 5% CO2 overnight.
The next day you will see the HFLU only growing next to the Staph. streak in tiny colonies. You can observe this in a mixed culture with both S.aureus and H.influenzae. On the BAP you will see tiny colonies satelliting around the Staph. colonies. You will see tan H. influenzae colonies growing on the chocolate only.
Please realize that HFLU needs both X and V. H. parainfluenzae only needs the V factor. We use commercially prepared X and V discs or strips and streak the suspicious colony on a BHI (brain heart infusion without blood) or TSA (trypticase soy agar without blood). Then we drop the X and V discs/strips about a 1/2 inch apart and incubate overnight. HFLU will grow BETWEEN the discs; H.parainfluenzae will grow around the V only. The colonies look similar on chocolate so this is the only way to differentiate.
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Clinical Microbiology since 1974
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| Malaysian
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Thanks so much bactitech.......and I'm going to say it again.....I ABSOLUTELY LOVE READING your posts.....they're so interesting I guess the expereince you bring with it is also an important factor.
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| bactitech
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Why thank you again, Malaysian. Maybe you're interested in going into ID?
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Clinical Microbiology since 1974
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| Malaysian
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ID.....identification....yes, I am.If your ID meant something else then kindly let me know!!And you don't have to thank me.......its me who needs to profusely thank you for sharing your knowledge and experience!!!!
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| bactitech
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ID=infectious disease. Sorry. If you like micro stuff, that may be the specialty for you.
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Clinical Microbiology since 1974
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| Malaysian
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I am interested in Pathology or Internal Medicine.......is Infectious Disease a branch of Internal Medicine or its a separate branch of itself?I actually love working in the lab. more than in the wards!
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| bactitech
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I think that infectious disease is a sub-specialty of internal medicine but since I'm not a doc, I don't know what requirements for residency are. I would guess you would have to complete IM first, then do a couple more years in ID.
There are two types of pathologists: anatomical and clinical. Clinical pathologists are the overseers of clinical laboratories, but in actuality, they're not in the lab doing testing. I work in a large lab and I never see our pathologists at all. Anatomical pathologists don't oversee laboratories at all, but just do what pathologists do, I guess, which is do gross on tissues and read the microscopic stuff, and do occasional autopsies. Forensic pathology is another subspecialty that requires further training. Dermatopathologists specialize in skin lesions and are in big demand.
As an ID doc, you would be seeing patients. ID docs get called in when the microbiological picture for the patient gets really ugly. Lab work is done by registered MT(ASCP) techs like me. Docs don't do it. Docs DO go into research, however.
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Clinical Microbiology since 1974
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| Malaysian
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Thanks.....for the input ecspcially for Pathology.....if clinical pathologists arent in the lab....then where are they and what are they actually doing?I suppose dermatopathology must be a sub speciality of pathology just like ID is of internal medicine.
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| bactitech
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They hang out with the other pathologists in the Pathology Department :-). Seriously, I think they do more of the management/administrative work of the Pathology departments, whereas anatomical pathologists do strictly tissue work.
Dr. Ed Uthman is active on a couple of lists I subscribe to and his web pages give a lot of information about a number of topics. Go here:
http://web2.airmail.net/uthman/net_resour/net_res...
for pathology information (he's a Clinical Pathologist, I believe).
Go here
http://web2.airmail.net/uthman/
for his extremely interesting home pages that cover many medical and non-medical topics (check out his RANTS! They're great!). He has a lot of links of interest for medical students, and many pathology images. If you're ultimately interested in Pathology, you might want to subscribe to a couple of the lists and see if the topics are of interest to you. The MEDLAB-L list is more lab oriented and I subscribe to that. Set to digests after subscription or you will get lots of email. I'm not sure what's in the Patho-L list as I'm not a subscriber to that list.
Internet newgroups are sometimes interesting (sci.med.laboratory or sci.med.pathology) although some of the lists are populated with outsider nutcases.
I found these sites on google - they might help you:
http://www.csmc.edu/pf_3087.html
http://www.csmc.edu/pf_5487.html
The second source gives a paragraph explanation for each pathology subcategory (anatomical vs. clinical).
I am registered through ASCP (American Society of Clinical Pathologists). Those four letters are the "gold standard" for getting hired in laboratories as a technologist. Here is their website:
http://www.ascp.org/
Hope this helps.
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| bactitech
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http://web2.airmail.net/uthman/rants/on_dermpath....
Thought you'd get a chuckle out of this article (those of you who may be considering dermatopathology).
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