rida
Forum Guru
Topics: 109
Posts: 721
|
Cloning of new eukaryotic gene was followed by insertion and ligation of the gene in an expression vector. The protein translated from this gene was then studied on a western blot and probed with 32PDNA yielding a positive result. These findings eliminate which of the following substances as a candidate for the likely gene product?
A) CREB
B) Protein Kinase A
C) RNA polymerase
D) SP - 1
D) Steroid receptor
___________________
"If He takes you to it, He'll take you through it."
|
| usmleasr
Forum Guru
Topics: 105
Posts: 970
|
b.protein kinase 
|
| rida
Forum Guru
Topics: 109
Posts: 721
|
You are correct. Can you explain?
___________________
"If He takes you to it, He'll take you through it."
|
| Malaysian
Forum Guru
Topics: 28
Posts: 778
|
Yeah,pls explain
|
| step12005
Forum Senior
Topics: 27
Posts: 122
|
'probed with 32PDNA' --> should be an DNA binding protein. Protein kinase A stays in the cytoplasm and catalyze other enzymes.
|
| rida
Forum Guru
Topics: 109
Posts: 721
|
thanx step12005, so i guess the probe only bings DNA binding proteins and protein kinase A is not one of them???
___________________
"If He takes you to it, He'll take you through it."
|
| step12005
Forum Senior
Topics: 27
Posts: 122
|
lol
|
| mehrdad
Forum Newbie
Topics: 2
Posts: 10
|
rida ithink u r right. protein kinase could be a recombinent protein and idetify by 125I -antibody
|
|
| |
| | | | | | | | |
