hschutza
Forum Newbie
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I have a few questions regarding E. coli.
1. Is a coagulase test something you would normally do to test for E. coli?
( I can't find if it's + or - anywhere on line!)
2. Is a coagulase test something you would normally do for any of the Enterobacteriaceae?
3. Is a coagulase test something you would normally do for a Gram-neg bacilli?
Searching in Seattle,
hschutza
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| bactitech
Forum Elite
Topics: 25
Posts: 512
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No - No - No
Coagulase testing is done on Staphylococcus. The organism must be a gram positive cocci and catalase positive. A low percentage of S. aureus are slide negative and tube positive. The tube test is the gold standard. However, most isolates are tested by commercial latex. Our lab uses Staphaurex but there are others. If the organism is slide negative but looks like S. aureus we proceed to a tube test using rabbit coagulase plasma. A 0.5 ml aliquot of plasma is inoculated with the suspect organism and incubated in a 35 degree C heat block for four hours. It is then looked at for clotting. If there is no clot, an additional overnight incubation at room temperature is performed. I had one the other day that did not clot at 35 but clotted overnight, so you just never know.
http://www.rlc.dcccd.edu/mathsci/reynolds/micro/l...
http://en.wikipedia.org/wiki/Coagulase
The reason you are still searching is because you won't find this test in relation to gram negs.
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Clinical Microbiology since 1974
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