clar697
Forum Guru
Topics: 190
Posts: 199
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Bacterial strain X is resistant to ampicillin and sensitive to kanamycin. Bacterial strain Y is resistant to kanamycin and sensitive to ampicillin. Bacterial strains X and Y are grown in mixed culture in medium without antibiotics, then the culture is plated on medium containing both ampicillin and kanamycin. Bacterial colonies grow on the plates. In a second experiment, Dnase is added to the mixed culture medium. When this mixed culture is plated on medium containing both antibiotics, no colonies grow. Assuming that bacterial cells are impermeable to Dnase, which of the following processes best explains these observations?
A) Conjugation
B) Mutation
C) Transduction
D) Transformation
E) Transposition
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| SpeaksSoftly
Forum Senior
Topics: 16
Posts: 190
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D
the only process that can be affected by DNAse is transformation
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| oxyrichwater
Forum Newbie
Topics: 0
Posts: 7
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Transformation means the "uptake" of DNA from the environment by the bacterium as against transduction(introduction of DNA by virus) and conjugation( by cell to cell contact).
DNAse destroys all extracellular DNA, so D is the answer
Transposition is a "mutation" (chromosomal segment is transferred to a new position on the same or another chromosome)
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