bactitech
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It's "Lyme" disease.
Where do you get the impression that one has to use a stain to diagnose this disease? It's usually detected serologically.
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| bioguy
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thats the difference between textbook and field knowledge. even if borrelia is diagnosed serologically in the lab, we have to learn that its Gram negative (textbook). i dont know if there are other stains.
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| bactitech
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http://www.buddycom.com/bacteria/nongram.html - info on Lyme.
Sorry about my curt answer. I posted that late at night after I worked and I was tired. 
Yes, I realize that there is an unfortunate disparity in what you learn in textbooks and what you see in your residencies. After having dealt with residents for over 30 years we just patiently hold their hands and lead them through their questions.
I'm not sure if Borrelia will stain with a gram stain or not. I guess it will stain faintly gram negative.
http://tinyurl.com/6c5zct - here is a Google image search
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| oxyrichwater
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i didn't know that.
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| SpeaksSoftly
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Well according to a book Borrelia can be visualized using analine dyes (Wright's or Giemsa stain) using light micropscopy.
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| Jackofknives
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darkfield
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| bioguy
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darkfield is for Treponema,
Giemsa stain - light microscope
Mnemonic for Giemsa stain - Try PlaCeBo
Trypanosomes, Plasmodium, Chlamydia, Borrelia
Edited by bioguy on 08/01/08 - 07:42 PM
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| Jackofknives
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Dudes, you have to realize that Giemsa stains everything with a chromatin. The problem resides whether or not it is useful.
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| bioguy
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of course we realize giemsa stains DNA.
There is no problem in understanding that it is useful in identifying Trypanosomes, Plasmodium, Chlamydia and Borrelia.
What you have to realize is that giemsa stained borrelia is visualized using light microscope and not darkfield.
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| Jackofknives
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What do think I am an idiot? of course you do not need staining for darkfield. the point is Borrelia is not clearly visible under LM even with staining such as Giemsa unless u use IHC, which is pointless not to mention very expensive and time consuming, thus making the OP question irrelevant. And it's not even considered Gram negative stained but rather Gram-negative enveloped, which is a big difference.
And I don’t get the fuss about the complain about textbook microb. Step1 pretty much only tests medical relevant facts that is essential in understanding the basics behind your every single choice in patient management, in contrast to some crap sh*t taught at some other places.
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| bioguy
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Do you feel like one? 
Relax, buddy! I might have misunderstood your one word post - "darkfield" in response to the above post.
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| SpeaksSoftly
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I got my info from FA 2007 edition.
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| bactitech
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FYI, Giemsa and Wright's stain are not used routinely in microbiology. The only thing we use Wright's stain for in micro is fecal smears for WBC's. Wright's is used in Hematology all the time.
Jack - sounds like you need some sleep 
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| drlilac2002
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Giemsa Stain
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| Passerby
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Giemsa Stain
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