NBME F5 S4 transposition

Prep for USMLE Forum

» Log in | Register

Forum

Step 1

Match

Prep4USMLE Forum » USMLE Step 1 Forum » Genetics Forum

NBME F5 S4 transposition

Author

8 Posts

clar697
Forum Guru
avatar

Topics: 190
Posts: 199

07/10/08 - 07:18 AM

A geneticist has artificially produced a microorganism that contains a new combination of genes. He wants to know if recombination or transposition has occurred. The major difference between these two processes is that recombination involves which of the following?

A) DNA repair
B) Endonuclease sites
C) Gene rearrangement
D) Homologous sites
E) Sites on the same chromosome

yousefreda
Forum Junior

Topics: 7
Posts: 85

07/13/08 - 07:36 AM

supra
Forum Elite
avatar

Topics: 19
Posts: 353

07/15/08 - 12:04 AM

shaphat1
Forum Newbie

Topics: 1
Posts: 11

07/15/08 - 11:44 AM

please explain...

DrNickSaysHello
Forum Newbie

Topics: 0
Posts: 13

09/26/08 - 03:10 PM

C? - Recombination causes gene rearrangement. Trasposition causes mutations that can result in changes in the amount of DNA in the genome.

alirizvi
Forum Elite
avatar

Topics: 22
Posts: 372

09/26/08 - 10:03 PM

I think its D are u guys sure its C ?

I thought recombination was when u take 2 bacterias that are non virulent or w/e and their chromosomal genomes are excahnge via homology to produce a progeny with NEW TRAIT.

Transposons can be C also because transposons are jumping genes and when these genes jump between chromosome and plasmid they can cause "re-arrangement of gene" think like VDJ segment in bacteria and transposon will jump J4 in front of J2 and thats will re arrange stuff.. i dnt know i cant really explain it but eh.

___________________
We are what we repeatedly do. Excellence, therefore, is not an act, but a habit. - Aristotle.

Mike99
Forum Junior

Topics: 11
Posts: 36

10/17/08 - 05:30 PM

How about B? Recombination is totally artificial. 1st we need to cut out fragment of DNA from whatever source using restriction endonuclease (EcoRI) than insert it into a vector to be able to replicate it Any thoughts???

brainstem
Forum Guru
avatar

Topics: 8
Posts: 653

11/23/08 - 04:01 AM

D

___________________
how high u go depends on how hard u try.

You don't have permission to post.