jean robert
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A 16 -year -old girl presents to her physician complaining of red ness and yellowish discharge from her left eye for the past 2 days. She reports minimal crusting upon awakening and denies eye pain or previous trauma. upon examination, there s diffuse conjunctival hyperemia associated with a mucoid discharge. The pupils dilate normally, although there is mild photophobia in the affected eye.Gram s stain of the exudate shows pleomophic, gram negative bacilli. To culture this organism with whole blood agar, which of the following must be used?
a) Antibiotics to inhibit normal flora
b) Cold temperature
c) Egg yolk cholesterol
d) Reduced oxygen tension
e) Staphylococcus aureus
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| grechita12
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a
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| GoodGirl
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Not an easy question.
Gnegative pleimorphic rod could be Hemophilus. Growth with Staph.aureus --> lyses blood agar--> release factors X and V .
Answer is E.
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| bactitech
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The answer is E. Hemophilus influenzae will not grow on blood agar without X and V factors. It will satellite around a Staph streak.
We now streak the organism on a TSA agar WITHOUT blood and apply X, V, and XV strips (commercially made) onto the plate and incubate overnight at 35 degrees in CO2. If the bug is H. influenzae, you will only see growth between the X and V strips and around the XV strip. If it is Hemophilus NOT influenzae, you will see growth around the V and the XV strips - not in between them.
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| basophilia
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E . H influenza , stafilococo satellite
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| bactitech
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"Staphylococcal satellite." 'Nuf said.
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| jean robert
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Well done !!
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