sigh
Forum Senior
Topics: 15
Posts: 152
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A clone of F+ E.coli, which possesses a Thr+ marker on its fertility plasmid, was produced in the laboratory. These bacteria were mixed with wild-type F- E.coli, which were Thr- under conditions that prevented binary fission but encouraged cell-to-cell contact. After 24 hours, the cultures were plated on threonine-deficient and threonine-enriched media, and the colonies were counted. What would be the expected result?
A. All clones would be F+ and grow on threonine-free medium
B. All clones would be F+ but would require threonine in their growth medium
C. Half of the clones would be F- and would grow on threonine-free medium
D. Half of the clones would be F+ and would grow on threonine-free medium
E. Half of the clones would be F+ but would require threonine in their growth medium.
Please expain your answer.
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| jean robert
Forum Guru
Topics: 145
Posts: 598
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A
That s conjugation. Conjugation is gene transfer from one bacterial to another involving direct cell to cell contact.
Please, correct me if i m wrong.
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| step1000
Topics: 10
Posts: 29
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what about the threonine part, Jean?
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| sigh
Forum Senior
Topics: 15
Posts: 152
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Yep, A is correct. What do you think about THR+/- ?
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