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Kaplan Qbank USMLE



Author16 Posts
  #1

The nucleotide sequence encoding four amino acids near the amino terminus of a 10- kd protein is 5´- ACT GAT TGC GTT -3´. This sequence is mutated by insertion of a single nucleotide (A) in the second codon and becomes 5´- ACT GAA TTG CGT -3´. Which of the following consequences of this mutation is most likely?

A ) Altered cell-cycle checkpoint

B ) Decreased rate of transcription

C ) Protein truncation

D ) Single amino acid substitution

  #2

C...

  #3

thanx crista

  #4

anne ,

I think that it will generate stop codon and thus protein will be truncated.What do you think ?

  #5

yes,correct

  #6

thanks smiling face

  #7

quite possible,bec of tt at ends.

  #8

i think D should be correct.
the substitution did not give any stop codon (UAG, UAA, UGA) or you guys assume that the stop codon will be generated on the complementary chain?

___________________
if you haven't any charity in your heart, you have the worst kind of heart trouble.

  #9

bobby,

What i thought is this :

:arrow: after substitution u get 5' ACT GAA TTG CGT 3'
:arrow: to read its antiparallel complementary ....(IAM READING IT FROM 3' ) UCG CUU UUC UGA...since UGA is stop codon..the protein will be truncated to 3 codons instaed of 4 .

Correct me if iam wrong thanks

  #10

asma,when i read it antiparallel,the complementary codon at the end appears to be AGU rather than UGA
:arrow: after substitution u get 5' ACT GAA TTG CGT 3'
:arrow: reading it during transcription,u get UCG CUU UUC AGU
so it doesnt turn out to be a stop codon.do correct me if i am wrong!!! :?:

  #11

i THINK IAM WRONG :cry: :roll:

ANYBODY PLZ HELP US :cry:

  #12

Correct me if i am wrong guys, but i thought that the template strand is 3 to 5 and then the coding strand is synthesized in 5 to 3, and its the same as the mRNA except the T's are swithced with U's. This is a coding strand, so it would be transcribed exactly the same as the given strand, except you would change all the T's to U giving you
5' ACU GAA UUG CGU 3' ????

___________________
"If He takes you to it, He'll take you through it."

  #13

hi helpneeded!i guess its the template strand bec the q says "The nucleotide sequence encoding four amino acids", and as its only the template strand which undergoes transcription,and then then translation of the transcribed mRNA.
do correct me please if i am wrong!!!

  #14

I see what your saying anne, thanx for clearing it up.... smiling face

___________________
"If He takes you to it, He'll take you through it."

  #15

Its the coding strand.....not the template strand

  #16

Rida, you are absolutely correct.
Insertion or deletion of a non-multiple of 3 in the DNA sequence will result in a frame shift mutation. So the protein encoded will be truncated prematurely (due to a possible stop codon down the road) or will result in an aberrant non-functional protein. Answer choice: C

KISS: Keep It Simple Simonne








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