macintosh
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I am havng a hard time understanding the gene markers aka fragment polmorphisms. I would like to know how these markers are introduced in the genome. Are these part of the exon. And what does repeating sequences mean?
Thank you for you help.
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| drgho
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Those are not introduced. We just tried to detect the disease gene by looking at their adjacent marker gene on assumption that the nearer the two genes, the more likely they will inherit together. Why use marker gene? reason is it is easier to detect the marker gene than the mutated disease gene.
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| macintosh
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I am sorry for not being clear. I meant to ask how the human genome evolved to contain the gene markers (or the repeating sequences as they are sometimes considered). I remember reading somewhere that based on an evolutionary standpoint these sequences are originally characteristic of a bacterial genome. Is it possible that these bacterial genes were initially introduced into human genome by bacteriophage (transduction-lysogenic conversion). If so, is it correct to refer to these as palindromes? Also do these gene sequences have an important function in human genome other than being used as markers in scientific labs. Do these get expressed to form mRNAs and ultimately form amino acids/proteins.
Additonally what does the term repeating sequences mean? Is it to say that these are inverted repeats like palindromes, or else that they are repeated many times over in a genome?
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| drgho
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Palidrome is the site for the restriction enzyme. Palidromic sequence in the marker gene is easily detected by RFLP. But, it may or may not be in the repeating sequence.
Not sure about the relationship between the bacteriophage and repeating sequences and their evolutionary derivation? Repeating sequence does have some important known fuctions like telomeric repeating sequence is important for meiotic division and controlling cell replication and senescence. Also centromeric repeating sequence for cell division. Hope this explains something.
Edited by drgho on 04/04/08 - 07:51 PM
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