macintosh Forum Elite
Topics: 38 Posts: 178
| | 04/03/08 - 02:42 AM  
 
   
 
|   #1 |
|
| drgho Forum Junior
Topics: 1 Posts: 114
| | 04/03/08 - 02:53 AM  
 
   
 
|   #2 |
I think E. It is lac operon. Without lactose, the repressor binds to the operator seq and inhibits the galactosidase. With lactose, lactose binds to repressor and inhibits its inhibtory effect. So at start, the galactosidase activity is high because of lactose in the medium and with time the lactose in the medium is metabolised, the repressor will bind to the operator sequence and inhibits the galactosidase.
|
| macintosh Forum Elite
Topics: 38 Posts: 178
| | 04/03/08 - 03:06 AM  
 
   
 
|   #3 |
E is correct. I found it confusing to read that the question mentions using a lactose medium for E coli, which by itself prevents the repressor from binding to the operator. This should increase beta glucosidase expression. Little did i realize that the activity is compared against time and is really asking what happens when lactose is not available.
|
|
| |
| | | |