bioguy
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during dna replication in euk cells, which occurs when topo I cleaves a PDE bond in 1 dna strand?
free rotation of 2 sections of dna helix on either side of nick
recombo event btw adjacent double helices
removal and subsequent transfer of small piece of dna to another position
removal of mismatched nucleotides that could result in delterious mutations
I think you are right - its uncoiling.
again you can eliminate all the other choices. they are not right for "Replication of DNA" in general.
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| bioguy
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don't know about the bacteriophage and the population genetics questions.
didnt' understand the choices of the Complement question
and don't know the cell junction question, will find it out soon.
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| hottie99
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yes i got the jist of the splicing, and the increased gene products, i will look up more if need be, thx for explaining, u are stellar for replying u thoughts on these q's, ur the bomb!! 
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| hottie99
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so the complement q answers were like in a list, the first columan is the 'bacterial killing' does it increase (or decrease as the choice was) or stay normal, and the second colum was 'generation of anaphylytoxins (same concept, inc/dec/nl) so we both said that yes we get that the anaphylytoxins would be normal bc since the defect is in the c6 component of complex, anaphylytoxins (c3a/c5a) are prior to complex so it would not affect that.. the problem i had was that when they mention that the antibody coated neisseria with the boy's plasma is incubated, what in the world is that doing?? and if so, why is bacterial killing ever going to decrease, isnt the point of that antibody like a vaccination, like giving antibody as vaccination, why would it decrease the killing of bacteria, isnt the point of vacc to kill the bugs so i dont get sick??? 
i get so confused, me and nbme have a secret luv-hate relationship 
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| bioguy
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several childrean who are members of hereditary def of C6 have neisseris induced sepsis. incubation of antibody-coated n. mengitidis with plasma from the affected children is most likely to produce which?
complement pathway upto C5 will be working - so you can generate C5a - anaphylotoxin
so that must be the answer.
you cannot generate Membrane attack complex - so no bacterial killing
but you still have C3b and IgG - opsonization - killing by phagocytosis.
oh- u already have the answer!
Edited by bioguy on 03/29/08 - 11:22 PM
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| hottie99
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cell junction i had answered zona adherens, bc that is at glandular apices, but study buddy pointed out that it asks 'basal' side, so on the basal side of those cells is basement membrane or hemidesmesome, right? crazy q's!!!
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| bioguy
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hottie99 wrote:yes i got the jist of the splicing, and the increased gene products, i will look up more if need be, thx for explaining, u are stellar for replying u thoughts on these q's, ur the bomb!!
hey, my exam is months away, so i am just sitting peacefully and thinking through the answers - and refering to Harrison's or other sources for confirmation. so don't praise me so much. i am no bomb.
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| hottie99
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yes but ur explanation is to the first part of the qustion rite?? like saying that the anaphylatoxins stay normal but backterial killing is decreased bc of lack of MAC complex rite?? i understand that, but i dont get the entire second statment, where the question is, like what isthe antibody coated bug going to do?? mabye do nothing?? so the killng is normal and anaphylytoxins is normal?? or shuld i not be paying attn to that part?
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| hottie99
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ok fine, ur not the bomb, i take it back, 
no i didn't have the answer, i was trying to figure out what the relevance was of that antibody, it is still driving me bananas!! so i was trying to 'type' outloud persay but still not so sure which it could be, i was only sure about anaphylytoxins being normal.
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| bioguy
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hottie99 wrote:
yes but ur explanation is to the first part of the qustion rite?? like saying that the anaphylatoxins stay normal but backterial killing is decreased bc of lack of MAC complex rite?? i understand that, but i dont get the entire second statment, where the question is, like what isthe antibody coated bug going to do?? mabye do nothing?? so the killng is normal and anaphylytoxins is normal?? or shuld i not be paying attn to that part?
you are right, this question is intense. the question was not clear in saying that they took the bug - coated it with antibodies (maybe IgG for opsonization or IgM for complement actiavtion) and then took the patients plasma (no blood cells) and mixed both in a test tube.
now you can answer the question.
since the plasma does not contain the cells (phagocytes) there is decreased bacterial killing. here you have to understand that antibodies by themselves don't kill the bugs. they assist in killing -
IgG - opsonization - killing mediated by Phagocytes
IgM - complement activation - killing mediated by MAC
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| hottie99
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hmmmm.... i see what ur saying.. gotta luv nbme and it's 'intensity' 
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| bioguy
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you know what? i am also going mad, trying to understand this complement question
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| bioguy
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the kids are already infected with N.m. sepsis. Does that mean anything?
whats the answer, by the way???
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| bioguy
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ok, buddy! good luck with your prep. i enjoyed answering these question.
but gotta go sleep.
take care.
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| hottie99
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thx so much for u help! anyone else got any input?? esp on the questions not attempted?? got any biostat luvers???
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