anne
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A bacterium harboring a 15,ooo bp plasmid that encodes resistance to tetracycline and ampicillin segregates mutants that lose resistance to either drug.each independent mutant plasmid appears to inc in size by approx 5200 bp.which best explains these findings/
a)formation of plasma concatmers
b)formation of pyrimidine dimers
c)homologous recombination
d)insertion of transposon
e)slip-stranded DNA replication error
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| jalabert
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insertion of transposon
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| anne
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could u please explain it?
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| jalabert
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The two things to consider are that 1) the plasmid started out at 15 kb, and ended up as 20 kb, the plasmid gained DNA some how and 2) the same increase in plasmid size results in two very specific mutations.
The only option that satifies these two conditions is the insertion of a transposon (5 kb gain of DNA) at two different sites that inactivates antibiotic resistance at the two sites of insertion.
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| anne
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thanx jalabert!
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| jalabert
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No problem
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| bobby
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:lol:
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