usmleasr
Forum Guru
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44. Double-stranded DNA can be denatured by heating and renatured by cooling. Renaturation under conditions of high stringency has which of the following effects?
A) Considerable base mismatching is permitted
B) GC base pairing is inhibited
C) GT base pairing is promoted
D) Mismatched DNA hybridization is prevented
i couldn't understand q ..what is stringent condition? is it energy def ?please explain...
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| asmi
Forum Hero
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yep  got it its D
see its telling under high stringent condition".--> prevents mismatching DNA hybridization
:arrow: .renaturation of complementary DNA strand is an imp step for probing( southern blot /PCR) --->" hybridization process"
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| jalabert
Forum Newbie
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D- mismatch base pairing is inhibited. The GC pairing must occur for reannealing to result.
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| asmi
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| krsma
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what is "a high stringent condition" :?:
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| Idiopathic
Forum Guru
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No mismatches, I believe. Stringency equates with accuracy.
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