GoodGirl
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An actively replicating cancer cell line with fluorescently labeled DNA is passed through a cell sorter and divided into three pools. Fifty percent of the cells contain a DNA content of N, 20% contain 2N, and the remaining 30% has varying amounts of DNA between N and 2N. Assuming that the life cycle of this cell line is approximately 20 hours, about how much time do the cells spend in S-phase on average?
A, 2 hours
B, 4 hours
C, 6 hours
D, 10 hours
E, 20 hours
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| GoodGirl
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No one ? Easy or hard, some one should answer. 
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| docdoc9
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10 hours????
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| pr20
Forum Senior
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No idea at all
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| GoodGirl
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Anybody else ?
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| GoodGirl
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The correct answer is C. The DNA content of the cells should be N during G1 or G0, 2N during G2 and M, and anywhere between N and 2N during S-phase, when DNA synthesis is actively occurring. Therefore, 30% of the cells are undergoing S-phase at any time. And because the life cycle of this cell line is 20 hours, 30% of 20 hours is equal to 6 hours.
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