icie
Forum Newbie
Topics: 1
Posts: 2
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Hi everyone 
I am new to this forum, and I'm so glad there is this forum for us to share our genetic knowledge! I have a question and not sure if the answer is correct. Please help!
Question--> The "sum" operon only exists in a very rare bacterium called Bacillus threethreefivis and consists of 2 genes encoding enzymes required to digest the sugar summerose (S1 and S2), their regulatory sequence, and a gene encoding the regulatory protein Reg. In an attempt to understand how this system works we have mutagenized a stock of B. threethreefivis and screened for inability to use summerose as a sole source of carbon. We isolated 6 such mutants: sum1-sum6. The region containing the operon is known, but unfortunately it is full of repetitive DNA, which makes it extremely tricky to sequence. So, for each of the 6 mutants, we decided to isolate that region, clone it into a plasmid and use the resulting constructs for a set of merodiploid studies.
Using the data presented below, try to come up with a model of how the sum operon works, and what is wrong with each of the mutants (ie what is the normal function of the DNA sequence that's affected in each mutant?).
Strain S1 and S2 activity with summerose S1+S2 w/o summerose
Wildtype high very low
sum 1 none none
sum2 very low very low
sum3 none none
sum4 very low very low
sum5 none none
sum6 high high
sum1/sum2 very low high sum1/sum3 none none
sum1/sum4 very low very low
sum2/sum4 very low high
sum2/sum5 very low high
sum4/sum5 very low very low
sum3/sum6 high high
wt/sum1 very low high
wt/sum2 very low high
wt/sum6 high high
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| icie
Forum Newbie
Topics: 1
Posts: 2
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this is my answer to it:
with sugar w/o sugar
S1 none none
S2 none none
Oc very low v. low
P none none
Reg
actually, I'm not even sure if I understand the question, I don't think my answer is correct. Please help~ 
thank you!!
-confused operon girl
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