anne
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A sample of purified oligopeptide sequence was treated with cyanogen bromide. The original sequence is NH3—Gly-Arg-Phe-Lys-Met-Asp-Tyr-Ala-Arg-Met-Glu—COO— Which of the following is the appropriate cleavage product?
A. Gly-Arg-Phe-Lys-Met/Asp-Tyr-Ala-Arg-Met/Glu
B. Gly-Arg-Phe/Lys-Met-Asp-Tyr/Ala-Arg-Met-Glu
C. Gly-Arg/Phe-Lys/Met-Asp-Tyr-Ala-Arg-Met-Glu
D. Gly/Arg-Phe-Lys-Met-Asp-Tyr-Ala/Arg-Met-Glu
E. Gly/Arg-Phe-Lys-Met-Asp-Tyr-Ala-Arg-Met-Glu
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| anne
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the ansis A,please explain to me if someone can figure out how this is the ans.
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| asmi
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it is a tough Q anne..but i remember someone explained it before..they said the enzyme/whatever will break at only Met group . :roll:
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| piter
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asmi look at the lippincott's bio, p 16.
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| asmi
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thanks peter will surely look at it .
wow they have given a nice diagram.... my fault for not reading biochem :oops:
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| merjr
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Was there any information that you can share asmi
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| krsma
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Well, In lippincott, the diagram indicates that Cyanogen Bromide cleaves a peptide at the C-terminal of Methionine.
Thus, the answer is A
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| merjr
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Thanks Usmle4me 
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