bobby
Forum Guru
Topics: 136
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normal flora dominant in the vagina of;
a new born deliverd by CS
girl infant
girl b/4 puberty
girl after puberty
girl with normal sex life
girl prof. sex worker
woman in menopause
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| oddissy4u
Forum Guru
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no flora in newborn by Cs
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| ferekide
Forum Junior
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so does anybody knows the answers?
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| bactitech
Forum Elite
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http://gsbs.utmb.edu/microbook/ch006.htm
Check out this link - scroll down. Gee guys, I found this on google in <1 minute and I'm OLD  ! You're young and much more computer literate than I.....
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Clinical Microbiology since 1974
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| ferekide
Forum Junior
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so what can we find in a girl's prof sex worker vagina?
more staph?and e coli...or the usual flora?
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hello...i am preparing for usmle 1
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| bactitech
Forum Elite
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The mind boggles with the possibilities. When we work up female genital specimens, the pathogens we look for are:
GC, Group B strep (because of the danger of passing this organism on to any newborn - the organism itself is not particularly a problem for the patient), yeast (usually is C. albicans), Gardnerella vaginalis (has been implicated in bacterial vaginosis; however, some labs do not work this up, as BV is a microscopic diagnosis - clue cells seen). Trichomonas can be seen from a wet prep, and Chlamydia can be diagnosed by other testing - not a bacterial culture. Nowadays this is usually done by DNA amplification testing. GC and Chlamydia can be done from the same sample swab. We do hundreds of these a week.
Anything else in a female vaginal specimen is pretty much lumped together as normal vaginal flora. We don't work up Staph. aureus UNLESS it is the predominant organism and nothing much else is growing. We also mention if Hemophilus sp. are present if they're there in large numbers without normal vaginal flora. GC is always identified regardless of number of colonies, as is Group B strep. Any beta hemolytic strep is typed and Group A is also reported, if present, as this can be dangerous in the female genital tract. I haven't seen it that often, however.
The only gram negative in a vaginal specimen that we look for is Shigella in children. A predominance of gram negative rods is ignored. We've had this discussion on the ASM's DivC list and virtually all micro labs ignore enteric gram negative rods. That said, we used to identify them in special cultures taken from the introitus of young pre-pubescent females seen by a urologist specializing in pediatric urology. We would work them up with urinary tract sensitivities as these were kids with numerous UTI's.
We reject culture requests for anaerobes on female genital specimens. About the only time we would perform one would be from an aspirate inside a uterus due to contamination from the vagina, where many anaerobes reside and are normal.
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Clinical Microbiology since 1974
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