oddissy4u
Forum Guru
Topics: 107
Posts: 389
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can someone explain hw to solve such ques ,wht r sticky ends n how to decide them?
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| bright_nnn
Forum Senior
Topics: 17
Posts: 97
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Pallindrome r sequences in the DNA where the Restruction endo nucleases act
Found easily Eg. A-T-C-C-T-A
sUPPOSE THIS IS ONE OF THE STRAND then if u note the Right Symmetrical is equal to the Left
This is a Pallindrome.If u cut this Pallndroe it will produce sticky ends
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| usmleasr
Forum Guru
Topics: 105
Posts: 970
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do u mean in single chain..?? :roll:
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| bright_nnn
Forum Senior
Topics: 17
Posts: 97
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Well As far as i know,
Paillindromes are symmetrical sequenes on Left and Right sides on BOTH STRANDS
Some restriction Enzymes cut this Symmetrically and some cut it Assymetrically.
The assymetrical ends in this Pallindrome is the Sticky end.
This Sticky end is used in BETTER COMINATION with a Vector DNA
and thus forms a Recombinant DNA
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| oddissy4u
Forum Guru
Topics: 107
Posts: 389
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does this eg show assymetrical?sticky ends?? :roll:
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| bright_nnn
Forum Senior
Topics: 17
Posts: 97
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A-T-T-G-G-C
T-A-A-C-C-G
This is a pallindrome.containing symmetry
A-T T-G-G-C
T-A-A-C and C-G
is ASSYMETRICAL and is used in DNA recombinant DNA
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| oddissy4u
Forum Guru
Topics: 107
Posts: 389
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thnx bright.
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