logitech Forum Newbie
Topics: 11 Posts: 14
| | 09/09/07 - 06:51 PM  
 
   
 
|   #1 |
psychotic, indigent man w/ a history of multisubstance abuse has been involuntarily hospitalized for 1 week. Because of persisten diarrhea, stools are sent for ova and parasites, revealingg numerous granular, spherical, thin-walles cysts measuring 10-20 mm in diameter. Trichrome stains show up to four nuclei in most of the cysts. These findings are consistent w/ which of the following organisms? A. Cryptosporidium parvum B. Dientamoeba fragilis C. Entamoeba histolytica D. Giardia lambia E. Isospora belli is it e.histoliica???/
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| amritt786 Forum Senior

Topics: 26 Posts: 199
| | 09/09/07 - 07:22 PM  
 
   
 
|   #2 |
think A
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| drgho Forum Junior
Topics: 1 Posts: 115
| | 09/10/07 - 03:19 AM  
 
   
 
|   #3 |
Entamoeba histolytica
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| bactitech Forum Elite

Topics: 25 Posts: 481
| | 09/10/07 - 09:25 AM  
 
   
 
|   #4 |
Do not think A. C. parvum is much smaller than 10-20 microns (NOT mm!!!). Measurements are everything with parasites. You MUST measure any potential parasite before even considering identification. C. parvum is too small, and the other parasites mentioned have <4 nuclei. Nuclei are also important in parasites. Another clue - C. parvum does not show up on trichrome staining. You need either a carbol fuchsin acid fast stain or fluorescent stain to see them. So...answer is E. histolytica.
___________________ Clinical Microbiology since 1974
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| hope4dabest Forum Elite

Topics: 16 Posts: 433
| | 09/10/07 - 10:49 AM  
 
   
 
|   #5 |
i guess E but not sure
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| bactitech Forum Elite

Topics: 25 Posts: 481
| | 09/10/07 - 11:20 AM  
 
   
 
|   #6 |
http://www.ufrgs.br/para-site/Imagensatlas/Protoz... http://www.dpd.cdc.gov/dpdx/HTML/ImageLibrary/Iso... This site gives measurements..... Apparently you guys are not getting the message. I. belli has thick walls and 2 nuclei. Key to parasite identification: MEASUREMENTS, number of nuclei, cyst or trophozoite form, patient history. You cannot make a parasite fit the wrong measurements. You cannot change the number of nuclei. Read the question thoroughly. http://www.dpd.cdc.gov/dpdx/ Check this CDC site - lots of great information. http://www.dpd.cdc.gov/dpdx/HTML/ImageLibrary/Ame... Entamoeba page - compares E. histolytica to E. coli - can be difficult for techs to differentiate between the two sometimes. This always shows up on our CAP unknowns. My 0.02.....
Edited by bactitech on 09/10/07 - 11:27 AM
___________________ Clinical Microbiology since 1974
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| unique1 InGodITrust

Topics: 19 Posts: 1,410
| | 09/10/07 - 12:32 PM  
 
   
 
|   #7 |
Thank you bactitech. I did not know that measurements are that important .
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| po Forum Elite
Topics: 39 Posts: 356
| | 09/11/07 - 02:03 PM  
 
   
 
|   #8 |
yes thank you for the links,very helpful.
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| hope4dabest Forum Elite

Topics: 16 Posts: 433
| | 09/12/07 - 11:52 AM  
 
   
 
|   #9 |
thanks, even i didnt know measurements and nuclei were so important
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