asmi Forum Hero
Topics: 1043 Posts: 4,609
| | 03/11/04 - 11:04 PM  
 
   
 
|   #1 |
A)Which one corresponds to 10 nm and 30 nm size.,which one is sensitive to nucleases and insensitive to nucleases . 1. euchromatin 2.heterochromatin B) histone helps in packaging of nucleosome: 1.H2 2.H3 3.H1 4.H4 C) which one relaxes + supercoils and which one relaxes negative supercoils. 1. topoisomerase I 2. DNA gyrase
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| usmle prep Forum Senior
Topics: 21 Posts: 100
| | 03/11/04 - 11:29 PM  
 
   
 
|   #2 |
H1 helps in packaging of nucleosomes.
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| kris2c Forum Senior
Topics: 30 Posts: 108
| | 03/12/04 - 01:37 AM  
 
   
 
|   #3 |
:idea: Which one corresponds to 10 nm and 30 nm size, which one is sensitive to nucleases and insensitive to nucleases. 1. Euchromatin ---> 10nm sensitive to "Nucleases" 2. Heterochromatin --> 30nm :idea: Histone helps in packaging of Nucleosome: 1.H2 * 2.H3 * 3.H1 -----> this is for tightening nucleosomes together 4.H4 * :idea: Which one relaxes (+) supercoils and which one relaxes (-) supercoils. 1. topoisomerase I ---> relax (-) supercoils 2. DNA gyrase or topoisomerase II ---> relax (+) supercoils uhm... :-k
___________________ Kris2c
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| usmleasr Forum Guru
Topics: 105 Posts: 970
| | 03/12/04 - 07:56 AM  
 
   
 
|   #4 |
i am not sure about super coils other two i agree with kris..H1 is linker DNA ..other 3 H2 H3 H4 form nucleo somes.. euchromatin is one expressed ..so has to be loosely bound...10nm fibers.. heterochromatin is condensed..30 nm fiber
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| asmi Forum Hero
Topics: 1043 Posts: 4,609
| | 03/12/04 - 11:26 AM  
 
   
 
|   #5 |
Thanks kris, You are absolutely right :icon_salut: Heterochromatin is insensitive to nucleases .
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| usmle prep Forum Senior
Topics: 21 Posts: 100
| | 03/12/04 - 08:12 PM  
 
   
 
|   #6 |
what is + supercoil? & what is - supercoil??
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