Jackofknives
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Anyone of you know stereoloy? or even better, worked in the field?
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| P4a99
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Stereology is an important and efficient tool in many applications of microscopy (such as petrography, materials science, and biosciences including histology, bone and neuroanatomy).
mmm, so you hope that would help you with your surgery dream?
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| Jackofknives
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I want to be a physician scientist and this is an inevitable tool and is getting increasing amount of attention due to its usefulness in the quantification of tissue in an unbiased manner and on a microscopic level. Stereological method is now required for acceptance in many high impact journals and increasing, though sadly not many ppl know how to wield this tool correctly.
I’m using it right now for my neuronscience research and I just wanted to see whether there are ppl here doing something similar.
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| SILVER
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Jackofknives wrote:
I want to be a physician scientist and this is an inevitable tool
so how exactly does this 'inevitable tool' work? i mean what exactly is stereology
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| Jackofknives
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Stereology in medicine is an interdisciplinary science based on stochastic geometry, probability theory, statistics and biology, as a way to estimate the parameters of irregularly shaped objects without introducing bias. It’s a methodological tool for obtaining information about 3D objects from 2D profiles. An example would be by systemicly selecting a small fraction of samples, less than 0.01% of the total tissue volume, you could estimate the morphology of cells such as in the brain the total # of neurons, glial cells, average cell volume, tissue volume, cell to cell interaction and more. In neurosurgery, this concept is applied in areas, such as in stereotaxis, neuronavigation and many other situations where prediction of 3D is necessary.
ref="http://www.lab.anhb.uwa.edu.au/mb140/scope/scopefrm.htm" target="_blank" Roman?>http://www.lab.anhb.uwa.edu.au/mb140/scope/scopef....
Pubmed has many good review articles and basic research papers as well, to name a few:
Gundersen, The efficiency of systematic sampling in stereology and its prediction.
Braendgaard, The total number of neurons in the human neocortex unbiasedly estimated using optical disectors
Y. Tang, A stereological method for estimating the total length and size of myelin fibers in human brain white matter
M. West, Stereological methods for estimating the total number of neurons and synapses: issues of precision and bias
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| P4a99
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I thought you might find this interesting (if you havent read it already):
R.M.P. by Ganong, chapter 32, page 618 ...
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| Jackofknives
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I usually dont read RMP, but sure I will check it out
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