usmleasr
Forum Guru
Topics: 105
Posts: 970
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A patient presents with retinoblastoma. He has a single tumor in
one eye. What test could be used to determine whether this is a
heritable or sporadic tumor?
A. DNA footprinting
B. Fluorescence-activated cell sorting (FACS)
C. Northern blotting
D. PCR
E. Western blotting
explain me the ans :oops:
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| usmleasr
Forum Guru
Topics: 105
Posts: 970
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some one help me :? :oops:
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| asmi
Forum Hero
Topics: 1043
Posts: 4,609
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i think its PCR/ FLUORESCENT INSITU HYBRIDIZATION.
http://www.geneclinics.com/profiles/retinoblastom...
IS THIS A BOARD Q ?
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| usmleasr
Forum Guru
Topics: 105
Posts: 970
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thanks asmi..i am confused while reading the article..... :oops:
anyway thanks for the ans....i need someone to teach me genetics :?
this q was in kaplan q bank....
hope such qs doesn't come for us... :wink:
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| Sakaki-
Forum Senior
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Posts: 238
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The Rb gene is a tumor suppressor gene, and the loss (or loss-of-function mutation) of this gene is responsible for retinoblastoma. It functions by binding to E2F.
For Northern blotting to be a wrong answer, that would mean that the Rb protein is expressed only at certain times during the cell cycle. When might that be?
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| asmi
Forum Hero
Topics: 1043
Posts: 4,609
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what i remember is that for retinoblastoma investigation of choice is PCR , southern blot ,FISH .
What is your answer usmleasr ?
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| usmleasr
Forum Guru
Topics: 105
Posts: 970
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yes i agree with u asmi..actually..Rb gene deletion leads to the disease..so tumour suppressor gene ( and hence its protein product is lost) ..implies..it is a loss of function mutation..so best way to analyze will be DNA analysis..which is done by PCR mostly... :roll:
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| black_tulip
Forum Senior
Topics: 14
Posts: 95
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yes, for disease with clearly point mutation or deletion, DNA analysis is a good method for diagnosis. Now, a number of AD, AR or X-linked disease can be detected by this way (Ref. FA-MCB)
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| black_tulip
Forum Senior
Topics: 14
Posts: 95
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PCR is useful in detecting the infections. it can amplifies trace amount of viral DNA or RNA after reverse transcription.
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