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Kaplan Qbank USMLE



Author11 Posts
  #1

hey......cud any one tell me difference between...or important differentiating features regarding these.....

generalised transduction
transposons
specialised transduction
transformation
conjugation
recombinations

  #2

well its like

in generalized transduction there is assembly error that means that when a phage enters a bacterium...it undergoes lytic cycle and every gene of bacterial chromosome has equal likely hood of enterin its genome...this phage then goes to other bacterium and integrates the gene in it

  #3

transposons are jumping genes...these are sequences of bases of chromosome or plasmids that can TRANSPOSE (translocate) from one part of chromosome or plasmid to another part within the same bacterium

  #4

usually involved in multiple drug resistence

  #5

in specialized transduction...

there is excision error..that means that when a phage enters genome..it undergoes lysogeny....after sometimes when there is some stimulus which breaks apart the repressor of transcription....there is breakdown of prophage from the chromosome of bacterium...if somehow the excission takes place at a wrong place...a part of bacterial chromosome enters the genome of virion....as the sequence is specific for a virus to enter into chromosome..its kinda specialized...

  #6

in transformation

there is naked DNA form which enters competent bacterial cells and then homologous recombination takes place

example is rough type S. Pneumonia can change to smooth type if there is NAKED DNA available in the solution in which they are grown...

  #7

bacillus spp H. influenzae, N. gonorrhoea, S. pneumonia are natural transformers...reminded me of the new movie TRANSFORMERS...did anybody see it? :P

  #8

in conjugation there is physical contact between the two bacteria and there is sex pilus involved which actually transfers genetic material from one bacterium to other...

a single strand is transfered



  #9

there ar etwo types of recombinations

site specific and homologous'

in site specific recombination which takes place when prophage is formed, when fertility factor is transferred, or when transposon is incorporated...a specific site of chromosome is incorporated with the base sequence

while in homologous recombination...there is exchange of base pairs involving recombinase A, B etc and there is no site specification except that the site of exchange should be homologous partially

  #10

THANKS A LOT ................SmokyWaters
i can see a lot of smoke in the forum.....nod

i went thru books when no one answered....
anyways revision is no bad.....
very well explained.coolcool

  #11

hehe...thanks for the appreciatoin although I was quite brief through the terminologies... smiling face







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