Ligase
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Hello everyone!
I am a computational chemist modeling a reaction mechanism of a bacterial ligase. This reaction involves fist a reaction between ATP and carboxylic group of UDP precursor resulting in a phosphate trasfer and acylphosphate formation. Next the condensing amino acid reacts with the acylphosphate resulting in a new amide bond formation which is accompanied by phosphate release.
As I would like to compare my model results with the experiment I need to calculate the activation energy from experimental published kinetic data. The published data comprise vmax and Km values measured towards ATP UDP precursor and amino acid. My background in Biochemistry in not particularly extensive any help or suggestion would be most welcome.
Thanks in advance 
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