Clozapine
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An uncommon serotype of a non-lactose fermenting, motile,gm -ve rod was found by laboratories in the health departments of adjacent states. In TSI agar media the organism formed an alkaline slant and blackened butt. The isolates were all from a small geographic area on either side of the border between the states, suggesting a common source for the isolates. (All the isolates were from otherwise healthy young adults who smoked marijuana; the same bacteria was isolated from a specimen of the marijuana.) By what method did the laboratories determine that these isolates were the same?
(A) Capsular (K antigen) typing
(B) O antigen and H antigen typing
(C) DNA sequencing
(D) Sugar fermentation pattern determination
(E) Decarboxylase reaction pattern determination
Edited by Clozapine on 01/22/07 - 08:03 PM
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| Palaniappan
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I am thinking of Proteus - Motile,Non-lactose fermenting,gram negative rod,producing H2S in TSi agar and probably resides in marijuana?!....However,can't rule out salmonella outrightly..
Answer B??
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| Clozapine
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Bingo 
Just wanted to let everyone know that Marijuana act as a source of infection for Non- S. typhi salmonella ( Salmonella enteritidis and S. typhimurium etc)
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| Clozapine
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Not K antigen b/c salmonella capsular antigen is known as Vi antigen, not K
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| Ancylostoma
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Shyps
oxidase - lactose - organism
Shigella non motile no h2s
yersina non motile no h2s
proteus motile h2s
salmonella motile h2s
Finally lets add to the questions, the H and o antigens Of proteus, are used to diagnose which disease ?
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| Clozapine
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H and O ? is it the same as OX Antigen ? Rocky mountain spotted fever?
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| Ancylostoma
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rememeber they are enterobactriacae , thus H is flagellar antigen, O is outer membran component. Ox refers to the O antigen from the OX subgroup of proteus which is serogroups (1-3). I accidently put H in and it has no relevance in dianosing rocky mountain spotted fever.
once last thing what is the unusual characteristic of the rash?
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| Clozapine
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Centripetal distriution
Palm and sole involved (others causing palm and sole inv ~ Syphilis and Coxsackie A~ HAnd foot mouth Disease )
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| Clozapine
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another Q
What are the urease positive organisms except proteus?
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| Palaniappan
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I can think of H.pylori and Yerisinia...Anything else??
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| Clozapine
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Yersinia  I didn know that ... only knew Yersinia was coagulase positive
H. pylori totally right
ureaplasma urealyticum
and also Culture of cryptococcus neoformans yields urease positive yeasts
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| Ancylostoma
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You guys will find this mnemonic useful
urease += Punch
Proteus
urea plasm urealyticum
Nocardia
Crytococcus
H pylori
I believe klebsiella is one too .
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| Palaniappan
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You are right clozapine..Yerisinia is only coagulase positive..NOT UREASE Positive
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| Clozapine
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PUNCH Awesome ... thanks Ancylostoma
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| Clozapine
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Corollary to Q 2 ,
MOTTS can be found in cigarettes
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| bactitech
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http://www.flashcardexchange.com/flashcards/view/318583
Learn the IMViC reactions instead of lists of one reaction. It makes much more sense! This seems to be a cool site. I forwarded it to our MT Student Coordinator.
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| bactitech
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Where did you get that Yersinia was coagulase positive??? Coagulase testing is only used on Staphylococcus sp. to differentiate between S. aureus and other coagulase negative Staph. sp. It is not used on gram negatives.
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| Clozapine
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Thanks bactitech .... Kaplan says Yersinia is coagulase positive .... might not be used as a significant test/reaction ... but I guess , it`s just another random piece of info the boards might test us about... as far as I remember, it has something to do with the mouth of the flea  ....
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| bactitech
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I'd like to know what micro lab these Kaplan people ever worked in. I have NEVER ever heard of coagulase as a test for gram negative rods. What about the mouth of a flea? I don't understand that reference at all.
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| Clozapine
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Bactitech, u r absolutely right. Y. pestis being coagulase positive is not important for diagnosis at all. It just helps in the disease transmission.
When a flea feeds on a rodent infected with Y pestis, the ingested organisms multiply in the foregut (proventriculus) of the flea and, helped by the coagulase, block its proventriculus so that no food can pass through. Subsequently, the "blocked" flea gets hungry and bites around ferociously, and the aspirated blood, contaminated with Y pestis from the flea, is regurgitated into the bite wound.
Hope I cud make it clear.
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