dxtxpx
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A dx of malaria was made in the case of 23 yr female who recently immigrated to US from Vietnam. During tx of parasitic infection she developed shaking chills and spiking fever. Several days later, small clusters of vesicles appeared around her mouth and nostrils
I) Mostl likely cause of facial lesion is
a) Coxsackie B virus
b) Herpes simplex virus
c) Varicella Zoster virus
d) Variola virus
e) Vaccinia virus
II) Most likely site of latency for this virus is
a) dorsal root ganglia
b) sacral ganglion
c) trigeminal ganglion
d) adenoid tissue
e) cornea
III) One method for distinguishing different viruses of same gp could include studying
a) inclusion formation within infected tissues
b) neutralizing antibodies
c) size of vesicles
d) location of vesicles
e) age of pt
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| asmi
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1.b
2.a
3.a
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| dxtxpx
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Only 1 is correct
Try 2&3 again
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| asmi
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2.c
3.B THOUGH D can also be a choice :?:
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| dxtxpx
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yes
I) b
II) c
III) b
The size, location and patterns of herpes virus lesions are indicative of different infections. Broad variations occur among individuals. Likewise, their ultrastructures are similar and not easily distinguished. A simple distinguishing procedure would be the use of neutralizing antibody. Recently, the use of nucleic acid probes and DNA fingerprinting techneques have been used to id herpes viruses
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| Malaysian
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why is the answer not dorsal root ganglia......i think the herpes simplex resides there???Also I wanted to know does the VZ virus also reside in the dorsal root ganglia?What about polio V. and rabies.....where do they reside?
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| mani
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think about the location of vesicles in this case and the cutaneous supply of that region. things will be clear
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| Bio
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The right answers are
1. B
2. C
3. B
or D (HSV 1 latent infection, trigeminal ganglia; HSV2 lantent infection, Sacral and Lumbra ganglia)
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| bactitech
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Herpes viruses are grown and identified every day in Virology labs all over the country via the use of culture. We get cultures on vesicles of various types all the time, especially for Herpes. You guys really like to spend money on expensive techniques (nucleic acid probes/DNA fingerprinting, etc.)
In the real world of real medicine, your patients' insurance companies won't allow you to spend money this freely :-).
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Clinical Microbiology since 1974
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| Bio
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A cell culture would only give you a presumptive diagnosis, and you can't distinguish between HSV-1 and HSV-2 based on cultures; they are structurally and morphologically identical. The most common means of telling them apart is either using Antigenicity and Restricted endonuclease patterns.
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| bactitech
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The cells are stained with monoclonal antibodies and typed for 1 and 2, so they are able to be distinguished that way. I can look up the procedure if you'd like. I don't perform it myself. The doctor has to request typing in some labs, I believe.
I will check with our Virology people tomorrow when I go into work.
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| bactitech
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http://www.labcorp.com/datasets/labcorp/html/chap...
http://www.aruplab.com/guides/ug/tests/0065065.js...
Here are the procedures for two of the biggest reference labs in the US (LabCorp and ARUP). Typing is included.
http://www.chemicon.com/Product/ProductDataSheet....
Here is one kit's information. I found this through google. I don't know what kit our lab uses. This one is a monoclonal antibody and is pretty typical.
http://www.herpesdiagnosis.com/diagnose.html
This site gives LOTS of information.
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Clinical Microbiology since 1974
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