 |  IgM ELISA ??
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| Author | 3 Posts |
gypsyeyes
Forum Newbie
Topics: 1
Posts: 3
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Hello,
Everyone, I am new here.
I would just like to know the advantages to using an IgM ELISA over IgG. Why use acute and convalescent samples? TO my understanding acute = during symptoms or within 3-14 days of invection and conv = recovery phase
Is an IgM elisa only used for diagnosis of diseases that are only symptomatic once per lifetime (west nile). Please Indicate results (ie what is happening when the acute is pos and the conv is neg, vice versa or they are both positive) I am having trouble understanding this.
Thank you in advance for your help!
gypsy
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| ManuNastai
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gypsy, it depends on the disease you are talking about... some diseases exist only in the acute form. some diseases don't develop an acute phase, they evolve subclinically as cronic diseases. most of them have an acute phase w/ more or less agressive simptoms and after that an cronic phase. they can be stopped and eradicated in the acute phase, preventing entering in a cronic phase (some of them) and some dispite correct treatment, progress in a cronic phase. in the cronic phase, the treatment is not any more curative (i.e. it doesn't cure the disease), it is paleative (i.e. it stops or SLOWS down the evolution).
now, regarding IgM and IgGs:
some diseases (mainly the infectious ones) stimulate the aquired immune system, yielding in a sinthesis of antibodies against the foreign organism. remember, some organisms don't do this, for they pass arround the immune system, tricking it (e.g. TB). first of all, durring the ACUTE phase, the IgM antibodyes are synthetised (you can understand this by studying immunology books) because of all those stuff w/ splicing of the mRNA carrying information for the heavy chain of Igs, because the miu gene is first in the row etc etc
after a while (which can be in the ACUTE or the CRONIC phase) the B Cell undergoes class swiching and transforms into a plasma cell which secretes IgG, IgE or IgA.
thus, if you wanna track the stage of a disease which follows the classic path which I wrote above, first you can't find the Abs (because they weren't synthetised, yet) only the Ags, after that the IgM, after that the IgM and IgG and after that only IgG and after many many years none of them
I hope this helps, 'cause it took me a long time 
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| gypsyeyes
Forum Newbie
Topics: 1
Posts: 3
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Thank you so much!
It helps a great deal and is very greatly appreciated
Have a great day!
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