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Kaplan Qbank USMLE



Author4 Posts
  #1

Bacterial strain X is resistant to ampicillin and sensitive to gentamicin. Bacterial strain Y is resistant to gentamicin and sensitive to ampicillin. Bacterial strains X and Y are grown in mixed culture in medium without antibiotics, then the culture is plated on medium containing both ampicillin and gentamicin. Bacterial colonies grow on the plates. In a second experiment, DNase is added to the mixed culture medium. When this mixed culture is plated on medium containing both antibiotics, no colonies grow. Assuming that bacterial cells are impermeable to DNase, which of the following processes best explains these observations?

A. Conjugation
B. Mutation
C. Transduction
D. Transformation
E. Transposition


this q was posted b4 and the answer many people agreed on was d. can anyone give me a nice explanation

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  #2

If the bacterial cells are impermeable to DNAase, it means that the actions of DNAase was at the level of the culture medium...hence it broke down fragments of DNA that was IN the culture medium.

Of the list, there's only one that involves bacteria picking up Nucleic material from its surrounding medium...

hope this helps.


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  #3

D?

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  #4

why not conjugation?







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