AngelT
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Anybody could explain me the answers of these 4 questions?
Thanks very much!
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| AngelT
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Questions 3 and 4
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| AngelT
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I am getting crazy wondering about these questions.. ; (
Please people..I am not good in Biochemistry..I need help!
Atleast questions 1 and 3..ok?
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| blueocean
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first q:I guess is glutamate because if it's possible to assume pH]=7 is physiologic pH than means arg, glu are + charged. Glu has a bigger molecular weight than so will migrate slowly.
second q: Go is negative which means the reaction could be in direction A to B but very slowly; a enzyme allows reaction to proceed rapidly.
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| blueocean
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Second file:
first q:Km means the concentration of a substrate at which the reaction velocity is 50 percent of the Vmax, so Km will be 0.5.
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| blueocean
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sec q[- A because AP is allosteric inhibitor of PFK1 which means ATP will be a modulator and will change the affinity of PFK1 for the substrate and the curve will be sigmoid.
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| AngelT
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Hi Blueocean!
Thanks for help! ; )
But the answers are:
1-B.arginine (I didnt understand because I also thought thats glutamate because its a negative charge)
2-G. cant be used for gluconeogenisis (because G< is irreversible?)
3-Right! Thanks..
4-B (but how could I know that ATP is an competitive inhibit..it could be noncompetitive inhibit as letter E..right?)
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| study_ing
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the marked amino acid is closest to the negativ epole...hence it is POSITIVELY CHARGED, in fact the most positively charged.
arginine, lysine and hstidine are basic
glutamate and aspartate are negativelty charged acidic.
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| study_ing
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angel it, idont have my glasses so im sorry im guessing at what u've posted.
the second rxn has a G which is negative. this shows its an energy liberating reaction...
actually which edition are thse notes..ill try looking at mine
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| study_ing
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3. asks for KM. look at the end of the second column for the maximum reaction velocity.
half it
and choose the correspondng substrate concentration
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| study_ing
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from what i remeber of chemistry, G duz NOT determine the RATE of enyme activity...its the energy of activation which helps predict that. ..in fact thats hpw enzymes used to work. they lowered the energy of activation reqd for a rxn. howevr the net energy yield or gain, denoted by g, remains unaltered. hence all the answers predicitng the spped of rxns wud be ruled out.
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| study_ing
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i think the graph shows allosteric inhibitoin..wait i'll get back to this.
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| study_ing
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was going over some images..will post them..remember graphs are supposed to be helpful..doi not get scared and they'll make life easy for u. y dont u try to post what u think each option in q4 represents and i'll tell u what it is
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| study_ing
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alloSteric= S shaped curve.
here's a site with q s and answers
http://www.biology.arizona.edu/biochemistry/probl... for energy of activation
http://www.biology.arizona.edu/biochemistry/probl...
for enzyme inhibition
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| AngelT
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Thanks study_ing!
I am using the 2001 edithion (Chapter 8-Aminoacids/Proteins and Enzymes) thats is similar from 2002.
Edited by AngelT on 08/28/06 - 02:38 PM
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| study_ing
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im sorry ive forgotten my biochem ..will try and come back to it later..
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| study_ing
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try posting 4 dif questions as 4 dif posts and try imaging them separately ( to get a clearer image) .. im sure u'll get more help that way..
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| AngelT
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Ok! Thanks for the site..i am reading it..
Edited by AngelT on 08/28/06 - 02:55 PM
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| AngelT
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I am asking about another question (Question 6)..could you help me on it also?
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