aminoacid

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aminoacid

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12 Posts

drpkaur
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08/18/06 - 04:29 PM

You have been given a task of purifying a protien suspected to be important in the regulation of insulin production. As a first step you seprate an extract obtained from the pancreas by Gel filtration chomatography. you monitor the eluted protein by measuring the abosorbant of the solution at 280 nm. you know that most of the absorbant of the protein at this wavelength is due to amino acid..

1..glutamic acid

2..tryptophan

3..serine

4..proline

5...alanine

vanshita
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08/18/06 - 04:45 PM

khorshid
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08/18/06 - 07:17 PM

1??

Korotkoff
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08/18/06 - 08:25 PM

I read somewhere that tyrosine and tryptophan absorb at approximately 280 nm

blueocean
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08/19/06 - 10:03 AM

tyrosine and tryptophan have 280 nm absorption.

docarchana
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08/19/06 - 10:04 AM

wats the ans kaurji

Cedrick
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08/19/06 - 03:32 PM

Cedrick
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08/19/06 - 03:32 PM

AngelT
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08/20/06 - 09:11 AM

4. proline???? .

vallia
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08/20/06 - 10:39 AM

#10

tryptophan

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drpkaur
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08/20/06 - 11:51 AM

#11

Yes trytophan is the answer....

drpkaur
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08/20/06 - 11:53 AM

#12

sorry for misspelling-tryptophan...

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