sridevibandaru24
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. A 16-year-old girl is febrile and incoherent but able to indicate that she has severe lower abdominal pain. Pelvic examination shows a foul-smelling cervicovaginal discharge, and a septic abortion is suspected. Which of the following procedures is most appropriate for identifying the microbial agents causing her infection?
A)Inoculation of anaerobic transport media at the bedside
B)Inoculation of the specimen into tissue culture
C)Quantitation of the bacteria in the discharge by using a calibrated inoculating loop
D)Streaking a swab specimen onto Thayer-Martin media at the bedside
E)Use of Löwenstein-Jensen and Sabouraud media for acid-fast bacteria and fungi, respectively
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| Dragonfly
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A.
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| docarchana
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can u plz explain the ans df. i dint get it.
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| Dragonfly
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well this is most probably a "Baterial vaginosis" (foul-smelling cervicovaginal discharge) which is caused by Anaerobe bact.s such as : Gardenella v. ,uroplasma u., bacteroid, provotella, etc.
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| docarchana
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thanx Df..
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| bactitech
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Unless the specimen is collected INSIDE the uterus during some sort of surgical procedure, culture for anaerobes on a vaginal discharge would be rejected by every laboratory I've ever worked at. Sorry to burst the Kaplan bubble....
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| Musuq
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so? answer?
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| bactitech
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By process of elimination, I think they want A as the answer, even though, in reality, this scenario would never happen in a clinical lab today unless they took the patient to surgery and aspirated purulent material for culture via syringe. A vaginal swab just doesn't cut it. They could request anaerobes, but would not get it from this type of specimen in our laboratory.
It would not be B, as a tissue culture is used for virus isolation.
It would not be C because there is no reason to quantitate growth in this type of specimen.
It would not be D because gonorrhoeae is not implicated in septic abortions. A TM plate is always set up on any genital specimen for culture regardless (in every lab I've ever worked in, anyway, and it's NOT done at the bedside).
LJ is used for mycobacteria, i.e. acid fast bacteria. AFB is not a likely pathogen in this site. Also, fungi are not usually the cause of foul smelling purulent discharge. Yeasts cause cheesy white discharge.
Hope this helps.
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Clinical Microbiology since 1974
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