A gene encodes a protein with 150 AA. There is one intron of 1000bps, a 5'untranslated region of 100bp and a 3' untranslated region of 200bp. In the final processed mRNA, how many bases lie between the start AUG codon and the final termination codon?
A. 1750
B. 750
C. 650
D. 450
E. 150
Please explain how do you calculate this...thanks
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Musuq Forum Guru
Topics: 103 Posts: 425
08/13/06 - 10:53 AM
#2
I am not sure, but I would do it like this...
The protein has 150aminoacids = 150(3bp)= 450bp
now they give us : Intron= 1000bp ; 5'UTR= 100bp ; 3'UTR=200bp
I wouldn't count Intron bp, because they will be spliced out during the process of transcription so, we would have
5'UTR--Exon--3'UTR
if the protein has 450bp that is also counting 3' and 5' UTR right? So finally
5'UTR (100bp) -- 150bp -- 3'UTR (200bp)
I am considering UTR as counted for the gene encoding this protein.
But I am not completely sure, please somebody correct me if I am wrong.
answer e) 150bp if UTR counts or
d) 450 if UTR doesn't count at all.
Vallia, where did u get this qs, does it have an answer? and thanks a lot for the questions
Edited by Musuq on 08/13/06 - 11:34 AM
DanVitor Forum Junior
Topics: 4 Posts: 34
08/13/06 - 10:59 AM
#3
I'll go with D because:
Only translated bp are counted>>> 150 x 3 =450bp And those are these that are coding for polipeptide.
vallia Forum Guru
Topics: 98 Posts: 889
08/13/06 - 01:05 PM
#4
it is D, the q was from Kaplan notes, thanks guys for explanations ___________________ fight possessed
Musuq Forum Guru
Topics: 103 Posts: 425
08/13/06 - 04:28 PM
#5
ok, then for sure is 450bp, thanks vallia, I haven't gone through all questioons from kaplan but it's good I know how to face thgis qs now, thanks guys.
AngelT Forum Senior
Topics: 30 Posts: 100
08/13/06 - 05:35 PM
#6
I agree! Its D.. 3x 150= 450..The other ones doesnt matter! ; )
mood1269 Forum Newbie
Topics: 1 Posts: 12
08/15/06 - 07:54 AM
#7
yes it is 450 ,
and i think it is the right answer be coz in the stem of q it says from AUG to TERMINATION CODE so the q excludes the untranslated regions .
mood1269 Forum Newbie
Topics: 1 Posts: 12
08/15/06 - 07:56 AM
#8
mood1269 wrote: yes it is 450 ,
and i think it is the right answer be coz in the stem of q it says from AUG to TERMINATION CODE so the q excludes the untranslated regions . and 150 aa x 3 bp for each aa = 450
Ig F Forum Elite
Topics: 3 Posts: 439
11/09/07 - 07:29 AM
#9
its 450.......itz a kaplanz question ___________________ i m not perfect but i wanna get close to it......
but it's good I know how to face thgis qs now, thanks guys.