polyclonal Ab against the variable region

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polyclonal Ab against the variable region

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3 Posts

pery
Forum Newbie

Topics: 1
Posts: 3

07/26/06 - 08:23 AM

I need help please.
I want to produce polyclonal Ab against the part of the variable region (CDR1 mostly) of human monoclonal Ab (IgG).
In my previous trials I've injected mAb into the rabbit and cleaned the pAb on G-sepharose column. Eventually I’ve got the non specific pAb population i.e. it recognizes many common IgG determinants.
I have very little quantity of the mAb so I cannot slice it in order to rid of the common parts (like Fc for example).
Please advice if there are ways to produce determinant specific anti-Ab antibodies (poly or mono).
Thanks in advance.
Pery

sigmadx
Forum Newbie

Topics: 1
Posts: 13

08/03/06 - 12:32 PM

Well, your antibody is only going to be as specific as your antigen. It sounds like you'll need to get a higher quanity of mAB that has been sliced and purified to contain mostly the CDR1 region.

In my experience, this is absolutely critical if you want a polyclonal Ab. If this doesn't work out, then I suggest going down the monoclonal path via injections into mice and then doing a hybridoma screen.

pery
Forum Newbie

Topics: 1
Posts: 3

08/07/06 - 07:38 AM

Thanks

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