Sybre
|
Hi,
I am a lab technicien in a university hospital in Sherbrooke, Quebec, Canada. We experience some problems whit the result of the routine coloration ( hematoxylin and eosin ). The problem is in the nuclei of the cells. There is the presence of some holes ( like a balloon ) in the nuclei. This problems is frequent whit the biopsy ( especially the prostatic ones ).
We could not find any link about this artefact. Is the problem chemical ? or is it because of the fixation ? We use 10 % formalin. Is it the dehydratation, that is not good enough ?
Some times, on two sections of the same block; one is OK and the other is full of nuclei « balloons ».
Can someone give me some informations to find a solution to our recurrent problem ?
Sylvie Breton
sylvie2223@sympatico.ca
CHUS, Sherbrooke, QUEBEC, CANADA
|
|
|
may be there is problem with the procedure(somewhere down the fixation or dehydrating)
You are getting few good slides too,this excludes the chemical as the cause of it.
You got to check it !
|
| asmi
Forum Hero
Topics: 1043
Posts: 4,609
|
It was me
i forgot to log in while answering.
Anyways check with the procedure of staining or the glass slides used.
|
|
| |
| | | |
