Musuq
Forum Guru
Topics: 103
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| Musuq
Forum Guru
Topics: 103
Posts: 425
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come on, nobody?
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| scion
Forum Newbie
Topics: 3
Posts: 19
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PKA.
All the others are DNA binding proteins, which produce positive results in gel shift assay as described in the text.
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| Musuq
Forum Guru
Topics: 103
Posts: 425
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B) PKA
The stem suggests that the protein isolated is a DNA-binding prot, since the probing was done with a DNA probe and not an antibody. PKA is an enzyme that phosphorylates other proteins rather than interacting with DNA, thus would give a negative result with a DNA probe. PKA is a cAMP dep kinase.
CREB (A) Stands for cAMP response element binding protein. This is a type of DNA binding prot which importantly links levels of the second messenger of nuemrous receptors (glucagon, Adenosin, some %-HT, ADH, TSH, etc) with expression of critical proteins
RNA Polym (C), key enzyme responsible for transcription. where TATAA seq at -25 and is the essential recognition site for the ez RNA polym
SP-1 (D), transcription factor with a ZINC FINGER MOTIF that controls gene expression in mammalian cells. It is said to function as a "housekeeping" factor, allowing other crucial enzymes involved with transcription to operate optimally.
Steroid receptor (E), also belongs to the superfamily of ZINC FINGER DNA binding prot. these characteristic fingers, wher Zn is bound to a pair of Cys and a pair of His, allow intimate interaction of the protein with major grooves in the DNA molecules. Such interaction is responsible for controlling gene expression and in turn, for assuring a steroid response in euk cell. An example would be CORTISOL and the induction of the ez phosphoenolpyruvate carboxykinase, resulting in increased hepatic gluconeogenesis and therefore Hyperglycemia.
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