surgeon99
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A
Complimentary and anti parallel
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| Musuq
Forum Guru
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no, try again
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| Ivil
Forum Senior
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D
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| star1
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C is the right sequence
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| scion
Forum Newbie
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C, is it right?
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| study_ing
Forum Fanatic
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A i think coz it is complimentary to C, the sequence revealed by electrophoresis.
discussion please
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| Cedrick
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I don't know but I will keep it in mind
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| unique1
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Musuq , I couldn't get this question. Please let us know the answer.
You have posted good questions. It will be very nice if you mention the source of the
q too as I am saving NBME for my assesment at the end of my prep and do not want to do any NBME qs at this stage. Thanx a lot.
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| raves
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this is a qbank question and the answer is B although i thought the answer would be D according to HY cell n molecular biology
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| vallia
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it is A, complementary and antiparalel
synthesized strand GACAGTAACGTCGT is written in 5'-3' direction (which is normal) and template strand would be 3' CTGTCATTGCAGCA 5' , written in 5-3 direction (by convention all base sequences are written this way) would be 5' ACGACGTTACTGTC 3'.
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| Musuq
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B)
The sequencing gel, when read from bottom (shortest) to top (longest), gives the sequence of the primer extension (choice D) T G C T G C A A T G A C A G
and hence the sequence of the strand complementary to the Coding Strand being sequenced.
The CODING strand being sequenced, would be complementary and antiparallel to the Primer extension ( choice D), now just rebuild your Coding sequence and verify that the sequence you give has to be in the 5-3 direction.
Primer: 5' T G C T G C A A T G A C A G 3'
Coding: 3' A C G A C G T T A C T G T C 5' <---- follow this direction
Note that an efficient strategy would be to identify the first nucleotide of the primer extension (a T on the gel) and deduce that the last nucleotide in the Coding strand would be an A. Only choice B ends with an A.
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| study_ing
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gos bless ya...keep up the good stuff
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| pallav
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no clues
kindly tell me the right answer
it really blasted my balls
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| actea1
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thanks for explanation , Musug.
I was trying to figure this question out, couldnot.. and I got it now
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| tolito
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good job musuq
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| Ig F
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guess wht it took me an hour to understand this...but finally i did.....i m happy..u r so cool musuq 
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| doc_shrutz
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THANX MUSUQ FOR A BRILLIANT QUESTION .... KEEP UP DA GOOD WORK ... GOD BLESS U
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