indidoc1
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HEY! r all still there?????
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| keepgoing
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nope we dont indidoc lets gv quick revision of chapter wise if we dont have any problem like TYPES OF DNA FOUND IN NATURE AND HOW THEY DIFFER?
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| keepgoing
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What phase of cell cycle dna replication take place?
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| Lily2008
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Sorry for the delay, a student have a Q for me.
Yes, A for my second question.
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| Lily2008
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S phase
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| keepgoing
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functions of topoisomerases?
where r u all?
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| indidoc1
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keepgoing, as dr lionel says.. types of dna in nature is not really tested in d exam but just fr info, there r 5 types... B,D,A,C &Z. we need to know only B-dna
n dna replication occurs in S phase
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| Lily2008
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Oh, no, I forget this one. looking for the book.
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| indidoc1
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topoisomerase 2(gyrase) introduces -ve supercoils while TOPO 1 relieves them
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| keepgoing
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yes its s phase...what is eucromotin and hetrochromatin?
dear lily and indidoc zade i saw focusmle online?where r u all?
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| zade
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I m here, but I am at work. I was trying to catch up with you guys. Good Q Lilly.
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| zade
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Euchromatin is loosely packaged and transcrip act.
Hetero is dense (tight) and inact.
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| indidoc1
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ok here's one of my Qs... an easy,basic one to start off
in human somatic cells, the no. of DNA molecules combining to form 1 chromosome in
1. interphase and
2. metaphase are
a 1 b2 c 3 d4 e5 f6
plz indicate ans as 1&2
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| keepgoing
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thanx indidoc but in KLN there are two types of DNA given well u never know what u get in exam..
well i was juz memorizing things from chapter 1 as there is nothing much difficult in chapter 1..so
well u ask questions or shall we move further to chapter next
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| Lily2008
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I have a question. What relations between relaxed DNA, negatively Supercoiled DNA and Positive supercoiled DNA?
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| indidoc1
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attempt that one. i'll explain n then move to another Q i made frm chap 1
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2
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| zade
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indidoc:
d answer to your last Q?
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| zade
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If I understood correctly it is 2.
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| indidoc1
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lily, i understood supercoiling by using 2 ribbons as Dna strands. as replication proceeds, strands open out n so so supercoiling goes on increasing. thats +ve supercoiling i.e. coiling in right handed way tightening d strand.
now, hold d ribbons till d part u have unravelled during replication. that part is relaxed DNA. the tightly coiled part is +vely supercoiled. hold tight \ly coiled part n cut it in b/w
then rotate d strands in direction opposite to tight coiling. now, u r DNA gyrase n introducing -ve supercoils to relieve +ve supercoiling.
its kinda diff to explain it in words. try it urself n u'll understand
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| keepgoing
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for lily2008
neg----if DNA is loosely wound than actual in WATSON-CRICK model
positive---id DNA more tightly wound
relax---when enzyme TOPOISOMERAES is added to unwind those supercoils by making transient breaks...
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| indidoc1
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i think u didn't understand my Q its in 2 parts..
1(interph) n 2(metaph) fr each part u hav to choose out of 6 options a-f
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| zade
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LillY:
DNA gyrase introduses negatively supercoil (looselu than watson) into relaxed DNA.
DNA Topoisomerase1 does the reverse.
Positeve supercoil is tighter than watson& crick.
Did that helpyou?
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| zade
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Indidoc:
Still not gettting ur Q.
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| zade
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Is it 2 & 4?
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