26malik
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A patient at a specialty clinic is undergoing evaluation for liver disease. Although the cause of the liver dysfunction is not known, the doctor suspects that the patient has a deficiency of a liver enzyme resulting from a novel deletion of the gene for the enzyme. Which of the following tools of molecular genetics would be most useful for studying this gene?
A. cDNA library
B. Fluorescence-activated cell sorting (FACS)
C. Genomic library
D. Plasmids
E. Southern blotting
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| robin082006
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C, but not sure
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| doc179
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E ?
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| general malaise
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E?
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| hellohie
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i think abt E,
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| 26malik
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The correct answer is E.
Deletions are routinely detected by two methods: Southern blotting (using a probe from either a cDNA or genomic library) or polymerase chain reaction (PCR) using primers specific for the gene. Both of these techniques will show size changes that will allow the investigator to determine the size of the deletion. Deletions may sometimes be detected by using restriction enzymes; for example, the loss of a normally present restriction site may indicate deletion of the DNA in the vicinity of that site.
Edited by 26malik on 05/24/06 - 10:08 PM
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| ashfaque
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E
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