ing
Forum Guru
Topics: 45
Posts: 435
|
Actively dividing fibroblasts in culture are transfered from a 37C incubator to a 0C ice bath, and 10-6(power) M colchicine is added to the culture medium. After 30 min, many cells have een arrested in mitosis. Which cytoskeletal protein is most likely to be involved?
A. Actin
B. Desmin
C. Dynein
D. Keratin
E. Kinesin
F. Lamins
G. Myosin
H. Neurofilament proteins
I. Tubulin
___________________
Never give up!!
|
| robin082006
Forum Hero
Topics: 471
Posts: 5,125
|
I?
I have just remembered that Colchicine, Vinbristine, Vinblastine act on tubulin.
___________________
The Key to Succeed is Patience.
|
| r_albayunen
Forum Senior
Topics: 0
Posts: 218
|
I
|
| drsnq
Forum Elite
Topics: 24
Posts: 168
|
I
___________________
drsnq
|
| surgery
Forum Junior
Topics: 0
Posts: 30
|
I remember Colchine acts on microtubule and we know that microtubule consists of Dyenin and Kinensin
So ing can u please put the right answer
|
| waqastariq
|
tubulin that forms the spindle fibers cannot get polymerized due to colchicine...
leading to arrest in metaphase of the M phase of cell cycle 
|
| tolito
Forum Fanatic
Topics: 119
Posts: 2,174
|
surgery, this is an NBME q and does not have an answer!!
waqastariq, i thought mitosis was arrested in anaphase by colchicine 
___________________
It has been a looooong hard journey but I am inches away from my destination...
|
| mjl1717
Forum Hero
Topics: 956
Posts: 5,452
|
As far as I know mitosis is arrested in metphase
___________________
Smell the coffee! "Is That an Osler move??"
|
| tolito
Forum Fanatic
Topics: 119
Posts: 2,174
|
ok. thanks for the correction
___________________
It has been a looooong hard journey but I am inches away from my destination...
|
| tolito
Forum Fanatic
Topics: 119
Posts: 2,174
|
well surgery, i am probably the last person to discuss this topic but here is what i can understand.
the major component of microtubules are tubulins (alpha and beta)
they have 2 motors, dyein and kinesin. dyein causes movement towards negative end while kinesin causes movement towards positive end.
in metaphase and anaphase, both kinesin and dyenin are involved.
colchicine to my knowledge acts on the tubulin itself and not the motor.
colchicine and vinca alkaloids bind to the spindle and prevent polymerization of the tubulin which inibits the spindle and arrests the cell in mitosis.
___________________
It has been a looooong hard journey but I am inches away from my destination...
|
| study_ing
Forum Fanatic
Topics: 180
Posts: 2,511
|
and for the sake of completion here r 2 relevant posts i liked on this toopic
PacKliTAXEL--> TAXANES keep the spindle PACKED, prevents spindle disassembly.
Vincristine and vinblastin
Believe in ONE( V for one) tubulin
i.e prevent assembly spindle assembly
by inhibiting polymaerization of tubulin Dimers (though)
these are obviously CCS (cell cycle specific) for the M phase
..
___________________
If you yourself are at peace, then there is at least some peace in the world.
|
| study_ing
Forum Fanatic
Topics: 180
Posts: 2,511
|
a KINd AuNT
KINesin ANTerograe transport
___________________
If you yourself are at peace, then there is at least some peace in the world.
|
|
| |
| | | | | | | | | | | | |
