DrVirgo
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82. Using reverse transcriptase and mRNA transcribed from the human glucokinase gene, a researcher constructs a single-strand cDNA. This cDNA will hybridize with which of the following sequences associated with the glucokinase gene?
A. An enhancer sequence
B. An exon sequence form teh coding strand
C. An intron sequence from teh template strand
D. The promoter sequence from the coding strand
E. The Shine-Dalgarno sequence form the template strand
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| doc179
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B
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| DrVirgo
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B is right.
Explanation?
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| doc179
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the cDNA is constructed here from the mRNA. mRNA is the result of transcription and splicing so it will be complementary to the coding strand and it will have only exons after splicing
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| DrVirgo
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Right, I agree.
I was wondering why not D?
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| doc179
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as far as I remember the promoter sequence does not get transcribed to the hnRNA. transcription starts at +1 position from the promoter I think. correct me if I am wrong.
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| doc179
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and even if it does , the promoter sequence would not be needed for the translation of the protein so that will be spliced out of the hnRNA to make mature mRNA
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| DrVirgo
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Thats true: No Promoter in the mRNA.
Thanks. 
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