DrVirgo
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14. A new selective estrogen receptor modulator is being tested to determine its effect on estrogen receptor-DNA binding. Using a polymerase chain reaction, the investigators want to amplify the DNA region where the estrogen receptor binds in order to study the effects of the drug. On an estrogen respnosive gene region, which segment of the DNA should be amplified for this study?
A. Region upstream from the promoter at -435
B. Region in the promoter sequence at -100
C. Region in the promoter sequence at -25
D. Region downstrem from the promoter at +1
E. Region downstream from the promoter at +25
(PLEASE EXPLAIN!) thanks.
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| DrVirgo
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Answer: A
(I think its because the HRE is upstream from the promoter)
Any other ideas?
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| lakshya
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the hormone response element binds to the enhancer region of the dna. The enhancer Dna sequence can be anywhere, before the promoter, inside any intron of the gene or after the 3' UTR region of the gene. Here the first choice matches with the enhancer site possibility, also the last one does. But stating that it is at +65 from the promoter site does not guarantee it to be in one of the introns, so the choice A is correct
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| robin082006
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good explanation, thanks
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| Daniel_Matos
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I would go with A as well...
B seems like it is the CAAT Box, though -100 is not the usual location of the CAAT Box...
C is the TATA Box, so it doesn't make much sense, for it is going to be the same sequence in most genes
D is the Methionine codon at position one, and it is pretty common
E, well, it is not likely for the enhancer to be after the promoter,
That's my 2 cents
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