USMLEGladiator
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1. Which of the following techniques is most accurate in showing that two bacterial DNAs are identical?
A. Hybridization
B. Northern blotting
C. Nucleotide sequencing
D. Polymerase chain reaction
E. Restriction enzyme analysis
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| mazinger
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A and J but not 100% sure
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| robin082006
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for question 1 why not C?
agree with J for the second q
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| USMLEGladiator
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Q1.ANS D?
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| gogetit
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for qn 1.. it is C..
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| doc179
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yeah...its C
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| mazinger
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you are probably right.. Im having trouble identifying the use of these techniques.. For Ex Southern Blots are for detecting DNA, Northern mRNA, Western proteins, SWestern transcription factors, and PCR for expanding short sequences, why did you pick d? well I probably answered my question.. duh, but I want to read it from someone else's point of view..
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| azirikani
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why not E.
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| doc179
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restriction endonucleases tell you only the site at which they are going to act they will not tell you abt the bases in between their sites of action
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| pearljam59
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PCR is AMPLIFYING a KNOWN piece of DNA from one piece to like a million!
Cooool.
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| ord
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It's A.
These hybrid segments of DNA can be used to determine the evolutionary relatedness of organisms by examining how similar or dissimilar the DNA base pair sequences are.
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| docsrinu
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wats the final ans - A or C?
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| mohelgamal
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I say C because in sequensing you can know the exact sequence of each others are all quantitaive assessment of relativety and so won't be as accurate
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| drfax
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C.
knowing DNA sequence is most effective way to compare similarity.
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| ssrpk
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among the options, other than C all are sort of short cuts, sequencing the whole DNA is time consuming and tiring and expensive effort but nontheless will give the most acurate information about the similarities and dissimilarities!
well as for hybridization, similar sequences won't hybridize at all! only complimentary ones do! even in complimentary, few base differences proportionally won't hinder the process!
answer is C
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