manu
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Streptococcus pneumoniae cells of genotype strsmtl− are transformed by donor DNA of genotype strrmtl+ and (in a separate experiment) by a mixture of two DNAs with genotypes strrmtl− and strsmtl+. The following table shows the results.
a. What does the first line of the table tell you? Why?
b. What does the second line of the table tell you? Why?
For the first part my answer is that the genes are not close to each other or even on different fragments, since double transformant strr mtl+ frequency is lower than the expected frequency assuming that the two genes are not linked.
I'm lost with the second part of the problem.
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| study_ing
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hmm any comments on this one?
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| study_ing
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anyone?
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| muzammil
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i think the table simply shows the results of two experiment the question tell us that the bacteria under study is Streptococcus pneumoniae cells of genotype strsmtl−
here are two experiements in one we doner is DNA of genotype strrmtl+ whose reaults are written in the first line
in second different experiment the donor is a mixture of two DNAs with genotypes strrmtl− and strsmtl+. and the pertanges of transformed bacteria we get from this experiment is wriitn in the second line
in both lines on the left sides are the donar and on the right the three number are the frequency of products obtained whoes genotyp is written on the top of right three columns
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| muzammil
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what do u say stdy_ng
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| study_ing
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thanx muzammil, lets post our views about the significance of the results.
1.using S(R) M(+) DNA in the first , we had a rate of transformation rate of 0.17 to S(R)M(+)
2.using a mixture of S(R) M(-) and S(S) M(+) thr rate to S(R) M(+) was much less
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| study_ing
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in either case S(R) was picked up more than M(-)
your turn muzammil
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| muzammil
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studying i agree with u in both experiment the rate of uptake of sr is more than m+ and the rate of uptake of both is the least
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| study_ing
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well what do we think about the implications of the result?
manu thought that 'the genes are not close to each other or even on different fragments, since double transformant strr mtl+ frequency is lower than the expected frequency ' ..and it sounds logical...what duz the second one convey?
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| muzammil
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one thi=ought just came tome is the probbalty of the cell being transformed using different genes from different dna segment is very less
as the combine of r and + is 0.0066 only
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| muzammil
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and the first dna segment of the mixture ie r and _ is used more often than the next ie s and +
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| muzammil
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i think the sencond line says that a cell will be transformes with only one segemnt and that r is taken up more often during transformation so the segemnt of strrm- is used more often than strsm+
what is ur result about the experiemnt
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