RayBerg
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Also, I'm a bit unclear about RFLPs, VNTRs, and polymorphism. I'd appreciate it if someone can clearify these for me
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| reet
Forum Fanatic
Topics: 152
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We do SOUTHERN BLOT analysis of RLFpS fr genetic testing.
RLFPolymorphismS that have arisen in humans over tousands of year evolution. MEACHANISMS that create it are--
a) MUTATION IN RESTRICTION SITES
b) expansion of REPETITIVE sequences within DNA for example -minnisatellites or VNTRS(Variable number of tandem repeats.
SO, southern blot is used to predict which members within family have inherited the ALLELE associated with the disease.
I JUSt collected info from KAPLAN,, but i need more input from others to clarify it more.
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| msyamp
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in genetic diagnosis. we use known genetic sequences just beside the diseased gene to be studied to map what happened to the gene.
RFLP is the amount of genetic material that is connect with a specific gene.
for example a diseased sckle gene may weigh 1.15kilobases
and normal may wiegt 1.35kbs
this difference is not because of the actual mutation. its just a single base pair mutation. the difference is because of the expression and after affects of the gene or it was inherited since generation with such genetic materia around it.
VNTR is due to nothing but tndem repeats at the end of chromosome.seen if the four famous disease. HD, FredericksAtaxia, MyotonicDystrophy, Fragile x
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