robin082006
Forum Hero
Topics: 471
Posts: 5,125
|
The nucleotide sequence encoding four amino acids near the amino terminus of a 10- kd protein is 5´- ACT GAT TGC GTT -3´. This sequence is mutated by insertion of a single nucleotide (A) in the second codon and becomes 5´- ACT GAA TTG CGT -3´. Which of the following consequences of this mutation is most likely?
A ) Altered cell-cycle checkpoint
B ) Decreased rate of transcription
C ) Protein truncation
D ) Single amino acid substitution
___________________
The Key to Succeed is Patience.
|
| cyra
Moderator
Topics: 29
Posts: 844
|
C
|
| LinaSarmiento
Forum Newbie
Topics: 1
Posts: 14
|
D. GAT(Asp) for GAA(Glu)
___________________
"Defy the old dragon, defy fear. The world may rage and quake but I shall remain singing in perfect peace." Johann Franck
|
| Geroo
Forum Guru
Topics: 114
Posts: 799
|
C.(TTG),stop codon.
|
| drk1980
Forum Guru
Topics: 147
Posts: 1,038
|
does TTG translate into a stop codon???
|
| LinaSarmiento
Forum Newbie
Topics: 1
Posts: 14
|
Stop codons UAG(TAG), UAA(TAA) and UGA (TGA): you are gone, you are away, you gone away...TTG or UUG is not a stop codon, it codes for Leucine... i think the answer is D. and in some many cases the third position is not revelant (it wobbles)...
___________________
"Defy the old dragon, defy fear. The world may rage and quake but I shall remain singing in perfect peace." Johann Franck
|
| Thunderball65
Forum Newbie
Topics: 3
Posts: 34
|
C - a frameshift leads to misreading of all nucleotides downstream resulting in protein truncation
but it is not a wobble since there is an inserted nucleotide
|
| roopashri
Forum Elite
Topics: 16
Posts: 190
|
its a frame shift mutation, leading to truncated protein
|
| Geroo
Forum Guru
Topics: 114
Posts: 799
|
sorry i was wrong
|
| sturge_weber
Forum Guru
Topics: 77
Posts: 1,042
|
i also go for c
|
| LinaSarmiento
Forum Newbie
Topics: 1
Posts: 14
|
oh yup it's C...i got it...
___________________
"Defy the old dragon, defy fear. The world may rage and quake but I shall remain singing in perfect peace." Johann Franck
|
| anjushree
Forum Guru
Topics: 64
Posts: 386
|
5'ACT GAA TTG CGT 3' -A coding strand
mRNA-5'ACU GAA UUA CGU 3'-new mRNA
original seq 5'ACT GAT TGC GTT 3'
mRNA 5'ACU GAU UGC GUU 3'
As there is no UAA,UAG,UGA -no truncated protein
3 a a substitution
can't change rate of transcription - as no seq of TATA or CAAT inv
ANS- Should be A
|
| msyamp
Forum Fanatic
Topics: 60
Posts: 1,462
|
genrally most of the frameshift mutations lead to truncated proteins.
___________________
If you think you can You can! If you think you cant you are right again!!
|
| msyamp
Forum Fanatic
Topics: 60
Posts: 1,462
|
still cant say why it cant be A. but truncation is the rsult of most frameshif mutations
___________________
If you think you can You can! If you think you cant you are right again!!
|
| sturge_weber
Forum Guru
Topics: 77
Posts: 1,042
|
well u took my words msyamp .. i was going to type the same, that is that hwnever we have a frameshift mutation, there will always be early truncation of the protein
|
| msyamp
Forum Fanatic
Topics: 60
Posts: 1,462
|
___________________
If you think you can You can! If you think you cant you are right again!!
|
| anjushree
Forum Guru
Topics: 64
Posts: 386
|
A new protein as it changes a no. of A.A .-------can lead to stop codon when further sequences are consider.
|
| msyamp
Forum Fanatic
Topics: 60
Posts: 1,462
|
yes that is why we have a truncated protein
see you have your answer
___________________
If you think you can You can! If you think you cant you are right again!!
|
| mazinger
Forum Guru
Topics: 46
Posts: 916
|
totally go for c... frameshift mutation
___________________
Ultraseven X
|
| malinda
Forum Guru
Topics: 162
Posts: 654
|
yup C
___________________
I CAN DO IT.
|
|
| |
| | | | | | | | | | | | | | | | | | | | |
