Tetracycline question

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Tetracycline question

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3 Posts

Nora

11/18/03 - 06:23 PM

Hey everyone.. I have a question that Im studying for an exam but cant find the answer and am having a tough time understanding. I hope this is the right place to post. Here goes... ok, some strains of bacteria are resistant to tetracycline while others are sensitive. How would I make an experiment to demonstrate that tetracycline resistance is an inherited trait encoded by DNA of the resistant strain and not the protein. This seems familiar with Griffiths mice experiment with pneumococci but Im just not sure. I think it has to do with transformation though... any suggestions on an experiment??

Julia
Forum Newbie

Topics: 1
Posts: 6

11/19/03 - 12:29 PM

Tis is the simplest one I can propose.

We may try to demonstrate transduction - transfer of a portion of DNA from one bacterial strain to another by means of viral infection. For this purpose we may lyse a tetracycline-resistant strain to obtain a virus, than apply lysogenate to the original culture. If this culture will be found to have become resistant - that is, DNA have been transferred.

juda

12/14/03 - 07:35 PM

i agree, in the process, restriction endonuclease is also used.

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